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@article{768344,
author = {Havliš, Jan and Mann, Mathias and Shevchenko, Andrej and Tomas, Henrik and Olsen, Jesper V.},
article_location = {U.S.A.},
article_number = {6},
keywords = {ASSISTED-LASER-DESORPTION/IONIZATION; POLYACRYLAMIDE-GELS; NANOELECTROSPRAY; MODEL; SILAC; MS},
language = {eng},
issn = {1745-2473},
journal = {Nature Protocols},
title = {In-gel digestion for mass spectrometric characterization of proteins and proteomes},
volume = {1},
year = {2008}
}
TY - JOUR
ID - 768344
AU - Havliš, Jan - Mann, Mathias - Shevchenko, Andrej - Tomas, Henrik - Olsen, Jesper V.
PY - 2008
TI - In-gel digestion for mass spectrometric characterization of proteins and proteomes
JF - Nature Protocols
VL - 1
IS - 6
SP - 2856-2860
EP - 2856-2860
PB - Nature Publishing Group
SN - 17452473
KW - ASSISTED-LASER-DESORPTION/IONIZATION
KW - POLYACRYLAMIDE-GELS
KW - NANOELECTROSPRAY
KW - MODEL
KW - SILAC
KW - MS
N2 - In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.
ER -
HAVLIŠ, Jan and Mathias MANN. In-gel digestion for mass spectrometric characterization of proteins and proteomes. \textit{Nature Protocols}. U.S.A.: Nature Publishing Group, 2008, vol.~1, No~6, p.~2856-2860. ISSN~1745-2473.
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