| BRINKMAN, Mary Beth, Melanie A. MCGILL, Jonas PETTERSSON, Arthur ROGERS, Petra MATĚJKOVÁ, David ŠMAJS, George M. WEINSTOCK, Steven J. NORRIS a Timothy PALZKILL. A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin. INFECTION AND IMMUNITY. The American Society for Microbiology, 2008, roč. 76, č. 5, s. 1848-1857. ISSN 0019-9567. |
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@article{768652,
author = {Brinkman, Mary Beth and McGill, Melanie A. and Pettersson, Jonas and Rogers, Arthur and Matějková, Petra and Šmajs, David and Weinstock, George M. and Norris, Steven J. and Palzkill, Timothy},
article_number = {5},
keywords = {Treponema pallidum; protective antigen;},
language = {eng},
issn = {0019-9567},
journal = {INFECTION AND IMMUNITY},
title = {A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin.},
volume = {76},
year = {2008}
}
TY - JOUR
ID - 768652
AU - Brinkman, Mary Beth - McGill, Melanie A. - Pettersson, Jonas - Rogers, Arthur - Matějková, Petra - Šmajs, David - Weinstock, George M. - Norris, Steven J. - Palzkill, Timothy
PY - 2008
TI - A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin.
JF - INFECTION AND IMMUNITY
VL - 76
IS - 5
SP - 1848-1857
EP - 1848-1857
PB - The American Society for Microbiology
SN - 00199567
KW - Treponema pallidum
KW - protective antigen;
N2 - The antigenicity, structural location, and function of the predicted lipoprotein TP0136 of Treponema pallidum subsp. pallidum were investigated based on previous screening studies indicating that anti-TP0136 antibodies are present in the sera of syphilis patients and experimentally infected rabbits. Recombinant TP0136 (rTP0136) protein was purified and shown to be strongly antigenic during human and experimental rabbit infection. The TP0136 protein was exposed on the surface of the bacterial outer membrane and bound to the host extracellular matrix glycoproteins fibronectin and laminin. In addition, the TP0136 open reading frame was shown to be highly polymorphic among T. pallidum subspecies and strains at the nucleotide and amino acid levels. Finally, the ability of rTP0136 protein to act as a protective antigen to subsequent challenge with infectious T. pallidum in the rabbit model of infection was assessed. Immunization with rTP0136 delayed ulceration but did not prevent infection or the formation of lesions. These results demonstrate that TP0136 is expressed on the outer membrane of the treponeme during infection and may be involved in attachment to host extracellular matrix components.
ER -
BRINKMAN, Mary Beth, Melanie A. MCGILL, Jonas PETTERSSON, Arthur ROGERS, Petra MATĚJKOVÁ, David ŠMAJS, George M. WEINSTOCK, Steven J. NORRIS a Timothy PALZKILL. A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin. \textit{INFECTION AND IMMUNITY}. The American Society for Microbiology, 2008, roč.~76, č.~5, s.~1848-1857. ISSN~0019-9567.
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