Detailed Information on Publication Record
2008
Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays
MATĚJKOVÁ, Petra, Michal STROUHAL, David ŠMAJS, Steven J. NORRIS, Timothy PALZKILL et. al.Basic information
Original name
Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays
Name in Czech
Určení kompletní genomové sekvence Treponema pallidum ssp. pallidum kmene SS14 s využitím oligonukleotidové array
Authors
MATĚJKOVÁ, Petra (203 Czech Republic), Michal STROUHAL (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), Steven J. NORRIS (840 United States of America), Timothy PALZKILL (840 United States of America), Joseph F. PETROSINO (840 United States of America), Erica SODERGREN (840 United States of America), Jason E. NORTON (840 United States of America), Jaz SINGH (840 United States of America), Todd A. RICHMOND (840 United States of America), Michael N. MOLLA (840 United States of America), Thomas J. ALBERT (840 United States of America) and George M. WEINSTOCK (840 United States of America)
Edition
BMC Microbiology, BioMed Central, 2008, 1471-2180
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.877
RIV identification code
RIV/00216224:14110/08:00024748
Organization unit
Faculty of Medicine
UT WoS
000256297900001
Keywords in English
Treponema pallidum ssp. pallidum; strain SS14; oligonucleotide arrays
Změněno: 25/6/2009 15:47, prof. MUDr. David Šmajs, Ph.D.
V originále
Syphilis spirochete Treponema pallidum ssp. pallidum remains the enigmatic pathogen, since no virulence factors have been identified and the pathogenesis of the disease is poorly understood. Increasing rates of new syphilis cases per year have been observed recently. The genome of the SS14 strain was sequenced to high accuracy by an oligonucleotide array strategy requiring hybridization to only three arrays (Comparative Genome Sequencing, CGS). Gaps in the resulting sequence were filled with targeted dideoxy-terminators (DDT) sequencing and the sequence was confirmed by whole genome fingerprinting (WGF). When compared to the Nichols strain, 327 single nucleotide substitutions (224 transitions, 103 transversions), 14 deletions, and 18 insertions were found. On the proteome level, the highest frequency of amino acid-altering substitution polymorphisms was in novel genes, while the lowest was in housekeeping genes, as expected by their evolutionary conservation. Evidence was also found for hypervariable regions and multiple regions showing intrastrain heterogeneity in the T. pallidum chromosome. The observed genetic changes do not have influence on the ability of Treponema pallidum to cause syphilitic infection, since both SS14 and Nichols are virulent in rabbit. However, this is the first assessment of the degree of variation between the two syphilis pathogens and paves the way for phylogenetic studies of this fascinating organism.
In Czech
Je popsáno určení kompletní genomové sekvence kmene Treponema pallidum ssp. pallidum SS14 s využitím oligonukleotidového čipu.
Links
| GA310/07/0321, research and development project |
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| MSM0021622415, plan (intention) |
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| NR8967, research and development project |
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