Detailed Information on Publication Record
2008
Image analysis of localization and translocation of apoptotic proteins in living cells
VAŘECHA, Miroslav, Jana AMRICHOVÁ, Michal ZIMMERMANN, Vladimír ULMAN, Michal KOZUBEK et. al.Basic information
Original name
Image analysis of localization and translocation of apoptotic proteins in living cells
Name in Czech
Obrazová analýza polohy a transportu apoptotických proteinů v živých buňkách
Authors
VAŘECHA, Miroslav (203 Czech Republic, guarantor), Jana AMRICHOVÁ (203 Czech Republic), Michal ZIMMERMANN (203 Czech Republic), Vladimír ULMAN (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic)
Edition
European Light Microscopy Initiative, 2008
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
Genetics and molecular biology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14330/08:00026068
Organization unit
Faculty of Informatics
Keywords in English
AIF;endonuclease G;AMID;living cells;colocalization;image analysis
Tags
Tags
International impact, Reviewed
Změněno: 2/6/2010 11:51, Mgr. Miroslav Vařecha, Ph.D.
V originále
We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in living cells using both single-cell colocalization image analysis and nuclear translocation image analysis. Image data were acquired using automated fluorescent microscope Zeiss Axiovert 200M with spinning disc confocal unit Yokogawa CSU-10. We confirmed the mitochondrial localization of endonuclease G and AIF by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF. This work was supported by The Ministry of Education, Youth and Sports of the Czech Republic (projects number MSM0021622419 and LC535).
In Czech
Obrazová analýza polohy a transportu apoptotických proteinů v živých buňkách.
Links
| LC535, research and development project |
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| MSM0021622419, plan (intention) |
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