Multidetection platform for microcolumn separation of proteins and peptides

PREISLER, Jan. Multidetection platform for microcolumn separation of proteins and peptides. 2008.

Basic information

• Original name: Multidetection platform for microcolumn separation of proteins and peptides
• Name in Czech: Multidetection platform for microcolumn separation of proteins and peptides
• Authors: PREISLER, Jan (203 Czech Republic, guarantor, belonging to the institution).
• Edition: 2008.

Other information

• Original language: English
• Type of outcome: Requested lectures
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Ireland
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/08:00033542
• Organization unit: Faculty of Science
• Keywords in English: CE; ICP; MALDI MS; interface; off-line coupling
• Tags: CE, ICP, interface, MALDI MS, off-line coupling

Abstract

This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/or mass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform. The developed instrumentation and methods will be tested on analysis of metal complexes, protein complexes with metals and phosphorylated proteins.

Abstract (in Czech)

This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/or mass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform. The developed instrumentation and methods will be tested on analysis of metal complexes, protein complexes with metals and phosphorylated proteins.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622411, plan (intention): Name: Studium a aplikace plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek

Investor: Ministry of Education, Youth and Sports of the CR, Study and application of plasma chemical reactions in non-isothermic low temperature plasma and its interaction with solid surface

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations