Detailed Information on Publication Record
2008
Multidetection platform for microcolumn separation of proteins and peptides
PREISLER, JanBasic information
Original name
Multidetection platform for microcolumn separation of proteins and peptides
Name in Czech
Multidetection platform for microcolumn separation of proteins and peptides
Authors
PREISLER, Jan (203 Czech Republic, guarantor, belonging to the institution)
Edition
2008
Other information
Language
English
Type of outcome
Vyžádané přednášky
Field of Study
10406 Analytical chemistry
Country of publisher
Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/08:00033542
Organization unit
Faculty of Science
Keywords in English
CE; ICP; MALDI MS; interface; off-line coupling
Tags
Změněno: 11/4/2013 09:44, prof. Mgr. Jan Preisler, Ph.D.
V originále
This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/or mass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform. The developed instrumentation and methods will be tested on analysis of metal complexes, protein complexes with metals and phosphorylated proteins.
In Czech
This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/or mass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform. The developed instrumentation and methods will be tested on analysis of metal complexes, protein complexes with metals and phosphorylated proteins.
Links
| LC06035, research and development project |
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| MSM0021622411, plan (intention) |
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| MSM0021622412, plan (intention) |
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| MSM0021622415, plan (intention) |
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