Detailed Information on Publication Record
2008
Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC
JEDLIČKOVÁ, L., D. GADAS, P. HAVLOVÁ and J. HAVELBasic information
Original name
Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC
Name in Czech
Stanovení ergosterolu v odrůdách ječmene a sladu v České republice pomocí HPLC
Authors
JEDLIČKOVÁ, L. (203 Czech Republic), D. GADAS (203 Czech Republic), P. HAVLOVÁ (203 Czech Republic) and J. HAVEL (203 Czech Republic, guarantor)
Edition
Journal of Agricultural and Food Chemistry, 2008, 0021-8561
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.562
RIV identification code
RIV/00216224:14310/08:00024771
Organization unit
Faculty of Science
UT WoS
000256445300029
Keywords in English
Ergosterol; Malt; Barley; Deoxynivalenol; Gushing; HPLC
Tags
Tags
International impact, Reviewed
Změněno: 1/7/2009 14:52, Mgr. Lenka Jedličková
V originále
In this study ergosterol levels in different varieties of barley and malt produced in the Czech Republic were determined. A modified high performance liquid chromatography (HPLC) method was statistically processed, validated (Effivalidation program) and applied to 124 samples of barley and malt. Ergosterol was isolated by extraction, saponification and the quantification was performed using HPLC with diode array detection (DAD). The content of ergosterol ranged between LOD-36.3 mg/kg in barley and LOD-131.1 mg/kg in malt. Ergosterol is presumably connected with metabolites generated when barley grain is attacked by pathogens and such barley often shows a high overfoaming (gushing) value. However, it was found that the content of ergosterol does not correlate with the degree of beer gushing.
In Czech
V této práci byl stanovován obsah ergosterolu v odrůdách ječmene a sladu pěstovaných v ČR metodou HPLC. Tato metoda byla statisticky vyhodnocena, validována a aplikována na 124 vzorků ječmene a sladu. Ergosterol byl izolován extrakcí, saponifikací a kvantifikován pomocí HPLC s DAD. Obsah ergosterolu se pohyboval mezi LOD-36.3 mg/kg v ječmeni a LOD-131.1 mg/kg ve sladu. Ergosterol je spojován s metabolity produkovanými pokud je ječmenné zrno napadeno pathogeny.Ječmen pak vykazuje vysokou hodnotu přepěňování. Přesto bylo zjištěno, že obsah ergosterolu nekoreluje se stupněm přepěňování piva.
Links
| GA525/06/0663, research and development project |
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