JEDLIČKOVÁ, L., D. GADAS, P. HAVLOVÁ and J. HAVEL. Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC. Journal of Agricultural and Food Chemistry. 2008, vol. 56, No 11, p. 4092-4095. ISSN 0021-8561.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC
Name in Czech Stanovení ergosterolu v odrůdách ječmene a sladu v České republice pomocí HPLC
Authors JEDLIČKOVÁ, L. (203 Czech Republic), D. GADAS (203 Czech Republic), P. HAVLOVÁ (203 Czech Republic) and J. HAVEL (203 Czech Republic, guarantor).
Edition Journal of Agricultural and Food Chemistry, 2008, 0021-8561.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.562
RIV identification code RIV/00216224:14310/08:00024771
Organization unit Faculty of Science
UT WoS 000256445300029
Keywords in English Ergosterol; Malt; Barley; Deoxynivalenol; Gushing; HPLC
Tags barley, Deoxynivalenol, ergosterol, Gushing, HPLC, Malt
Tags International impact, Reviewed
Changed by Changed by: Mgr. Lenka Jedličková, učo 77837. Changed: 1/7/2009 14:52.
Abstract
In this study ergosterol levels in different varieties of barley and malt produced in the Czech Republic were determined. A modified high performance liquid chromatography (HPLC) method was statistically processed, validated (Effivalidation program) and applied to 124 samples of barley and malt. Ergosterol was isolated by extraction, saponification and the quantification was performed using HPLC with diode array detection (DAD). The content of ergosterol ranged between LOD-36.3 mg/kg in barley and LOD-131.1 mg/kg in malt. Ergosterol is presumably connected with metabolites generated when barley grain is attacked by pathogens and such barley often shows a high overfoaming (gushing) value. However, it was found that the content of ergosterol does not correlate with the degree of beer gushing.
Abstract (in Czech)
V této práci byl stanovován obsah ergosterolu v odrůdách ječmene a sladu pěstovaných v ČR metodou HPLC. Tato metoda byla statisticky vyhodnocena, validována a aplikována na 124 vzorků ječmene a sladu. Ergosterol byl izolován extrakcí, saponifikací a kvantifikován pomocí HPLC s DAD. Obsah ergosterolu se pohyboval mezi LOD-36.3 mg/kg v ječmeni a LOD-131.1 mg/kg ve sladu. Ergosterol je spojován s metabolity produkovanými pokud je ječmenné zrno napadeno pathogeny.Ječmen pak vykazuje vysokou hodnotu přepěňování. Přesto bylo zjištěno, že obsah ergosterolu nekoreluje se stupněm přepěňování piva.
Links
GA525/06/0663, research and development projectName: Sledování chemických změn obilky ječmene po napadení patogeny pro kontrolu a zvýšení kvality ječmene a následných potravinářských produktů
Investor: Czech Science Foundation, Study of the chemical changes of barley caryopses after an attack by pathogens to monitor and improve the quality of barley and its derived food products
PrintDisplayed: 28/7/2024 23:20