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@article{770117,
author = {Stsiaspanava, A. and Koudeláková, Táňa and Lapkouski, M. and Pavlová, Martina and Damborský, Jiří and KutáandSmatanová, Ivana},
article_number = {64},
keywords = {haloalkane dehalogenases; enzyme DhaA; crystals;},
language = {eng},
issn = {1744-3091},
journal = {Acta Crystallographica Section D: Biological Crystallography},
title = {Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis.},
url = {http://loschmidt.chemi.muni.cz/peg/abstracts/acsf08.html},
volume = {F},
year = {2008}
}
TY - JOUR
ID - 770117
AU - Stsiaspanava, A. - Koudeláková, Táňa - Lapkouski, M. - Pavlová, Martina - Damborský, Jiří - Kutá-Smatanová, Ivana
PY - 2008
TI - Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis.
JF - Acta Crystallographica Section D: Biological Crystallography
VL - F
IS - 64
SP - 137-140
EP - 137-140
SN - 17443091
KW - haloalkane dehalogenases
KW - enzyme DhaA
KW - crystals;
UR - http://loschmidt.chemi.muni.cz/peg/abstracts/acsf08.html
N2 - The enzyme DhaA from Rhodococcus rhodochrous NCIMB 13064 belongs to the haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols. The haloalkane dehalogenase DhaA and its variants can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Three mutants named DhaA04, DhaA14 and DhaA15 were constructed in order to study the importance of tunnels connecting the buried active site with the surrounding solvent to the enzymatic activity. All protein mutants were crystallized using the sitting-drop vapour-diffusion method. The crystals of DhaA04 belonged to the orthorhombic space group P212121, while the crystals of the other two mutants DhaA14 and DhaA15 belonged to the triclinic space group P1. Native data sets were collected for the DhaA04, DhaA14 and DhaA15 mutants at beamline X11 of EMBL, DESY, Hamburg to the high resolutions of 1.30, 0.95 and 1.15 A, respectively.
ER -
STSIASPANAVA, A., Táňa KOUDELÁKOVÁ, M. LAPKOUSKI, Martina PAVLOVÁ, Jiří DAMBORSKÝ and Ivana KUTÁ-SMATANOVÁ. Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis. \textit{Acta Crystallographica Section D: Biological Crystallography}. 2008, F, No~64, p.~137-140. ISSN~1744-3091.
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