| EISELLEOVÁ, Lívia, Iveta PETERKOVÁ, Jakub NERADIL, Iva SLANINOVÁ, Aleš HAMPL and Petr DVOŘÁK. Comparative study of mouse and human feeder cells for human embryonic stem cells. The International journal of developmental biology. Spain: University Of The Basque Country Press, 2008, vol. 4, No 52, p. 353-363. ISSN 0214-6282. |
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@article{770132,
author = {Eiselleová, Lívia and Peterková, Iveta and Neradil, Jakub and Slaninová, Iva and Hampl, Aleš and Dvořák, Petr},
article_location = {Spain},
article_number = {52},
keywords = {hESCs; human embryonic stem cell; growth factor production; undifferentiated growth},
language = {eng},
issn = {0214-6282},
journal = {The International journal of developmental biology},
title = {Comparative study of mouse and human feeder cells for human embryonic stem cells},
volume = {4},
year = {2008}
}
TY - JOUR
ID - 770132
AU - Eiselleová, Lívia - Peterková, Iveta - Neradil, Jakub - Slaninová, Iva - Hampl, Aleš - Dvořák, Petr
PY - 2008
TI - Comparative study of mouse and human feeder cells for human embryonic stem cells
JF - The International journal of developmental biology
VL - 4
IS - 52
SP - 353-363
EP - 353-363
PB - University Of The Basque Country Press
SN - 02146282
KW - hESCs
KW - human embryonic stem cell
KW - growth factor production
KW - undifferentiated growth
N2 - Various types of feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting factors. Here, we compared the expression and secretion of four well-established regulators of hESC pluripotency and/or differentiation among five lines of human foreskin fibroblasts and primary mouse embryonic fibroblasts throughout a standard hESC culture procedure. We found that production of TGFbeta1, activin A, and FGF-2 varied considerably among the human-derived feeder cell lines. Low- and high-producing human feeder cells as well as mouse feeder cells were evaluated for their ability to support the undifferentiated growth of hESCs and for the expression of stem cell markers. Our results suggest that the ability of a feeder layer to promote the undifferentiated growth of hESCs is attributable to its characteristic growth factor production.
ER -
EISELLEOVÁ, Lívia, Iveta PETERKOVÁ, Jakub NERADIL, Iva SLANINOVÁ, Aleš HAMPL and Petr DVOŘÁK. Comparative study of mouse and human feeder cells for human embryonic stem cells. \textit{The International journal of developmental biology}. Spain: University Of The Basque Country Press, 2008, vol.~4, No~52, p.~353-363. ISSN~0214-6282.
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