Borrelia burgdorferi sensu lato a jiné spirochety v klíšťatech a komárech

NEJEDLÁ, Petra, Adam NOREK, Karel VOSTAL and Alena ŽÁKOVSKÁ. Borrelia burgdorferi sensu lato a jiné spirochety v klíšťatech a komárech (Borrelia burgdorferi sensu lato and other spirochetes in ticks and mosquitoes). In XVII. konference mladých mikrobiologů, Tomáškovy dny 2008. 2008.

Basic information

• Original name: Borrelia burgdorferi sensu lato a jiné spirochety v klíšťatech a komárech
• Name in Czech: Borrelia burgdorferi sensu lato a jiné spirochety v klíšťatech a komárech
• Name (in English): Borrelia burgdorferi sensu lato and other spirochetes in ticks and mosquitoes
• Authors: NEJEDLÁ, Petra (203 Czech Republic, belonging to the institution), Adam NOREK (203 Czech Republic), Karel VOSTAL (203 Czech Republic, belonging to the institution) and Alena ŽÁKOVSKÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: XVII. konference mladých mikrobiologů, Tomáškovy dny 2008, 2008.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/08:00026187
• Organization unit: Faculty of Science
• Keywords in English: Borrelia burgdorferi sensu lato; ticks; mosquitoes; DFM; PCR
• Tags: Borrelia burgdorferi sensu lato, DFM, mosquitoes, PCR, ticks

Abstract

Vyšetřili jsme klíšťata druhu Ixodes ricinus a komáry (dospělce, larvy) několika druhů pomocí DFM na přítomnost spirochet. Pozitivní vzorky byly dále vyšetřeny metodou PCR na přítomnost DNA Borrelia burgdorferi sensu lato. Klíšťata vykazovala vyšší pozitivitu na přítomnost borrelií než komáři. Mezi pozitivitou metod DFM a PCR byl značný rozdíl způsobený zřejmě různou citlivostí a/nebo přítomností jiných spirochet. Z komára druhu Culex pipiens se podařilo izolovat dva kmeny bakterií, z nichž jeden byl určen jako Borrelia afzelii (Bbsl), druhý je neznámý.

Abstract (in English)

We examined Ixodes ricinus ticks and mosquitoes (adults, larvae) of various species on the presence of spirochetes using DFM. Positive samples were examined for the presence of Borrelia burgdorferi sensu lato DNA by PCR method. Ticks showed higher Bbsl positivity than mosquitoes. There was signifficant difference between positivity of DFM and PCR caused probably by different sensitivity and/or by the presence of other spirochetes. Two bacterial strains were isolated from Culex pipiens mosquito, one determined as Borrelia afzelii, one is unknown.

Links

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations