The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae

BÜYÜKGÜZEL, Ender, Pavel HYRŠL a Kemal BÜYÜKGÜZEL. The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae. In SEB Book of abstracts. 2008.

Základní údaje

• Originální název: The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae
• Název česky: Vliv inhibitorů biosyntézy eicosanoidů na oxidativní stres, antioxidační odpověď a proteinové spektrum hemolymfy larev Galleria mellonella
• Autoři: BÜYÜKGÜZEL, Ender (792 Turecko), Pavel HYRŠL (203 Česká republika, garant, domácí) a Kemal BÜYÜKGÜZEL (792 Turecko).
• Vydání: SEB Book of abstracts, 2008.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 30105 Physiology
• Stát vydavatele: Francie
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/08:00042007
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: Galleria mellonella; esculetin; malondialdehyde; glutathione S-transferase; hemolymph proteins
• Štítky: esculetin, Galleria mellonella, glutathione S-transferase, hemolymph proteins, malondialdehyde
• Příznaky: Mezinárodní význam

Anotace

Effects of eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone on hemolymph concentrations of the oxidative stress indicator malondialdehyde (MDA), antioxidant enzyme (glutathione S-transferase GST) and protein profiles of greater wax moth, Galleria mellonella (L.) seventh instar larvae were investigated. The insects were reared from first instars on artificial diets containing 0.001, 0.01, 0.1 and 1.0 g EBIs per 100 g of diets. MDA content was significantly increased in the presence of esculetin. Low concentrations of esculetin led to increased GST activity. Dexamethasone at concentrations of 0.001 and 0.01% significantly resulted in increased hemolymph MDA content. However, high dietary dexamethasone concentrations caused significant decrease in MDA content while these concentrations resulted in increased GST activity by about three-fold. Dietary phenidone led to increased MDA content and GST activity in the larvae. The quantity of the 45 kDa protein fraction from total 16 protein detected was increased with increasing esculetin concentrations. An increase after phenidone and dexamethasone exposure was detected only at 0.001 and 0.01% concentration. Other main hemolymph proteins such as ApoLP-I and storage proteins were not altered in larvae reared on diets amended with the EBIs. We suggest that 45 kDa protein may be an oxidative stress-related protein in hemolymph of G. mellonella. This is the first demonstration that antioxidative responses to a EBI exposure are modulated by a physiological system that includes eicosanoid biosynthesis.

Anotace česky

Effects of eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone on hemolymph concentrations of the oxidative stress indicator malondialdehyde (MDA), antioxidant enzyme (glutathione S-transferase GST) and protein profiles of greater wax moth, Galleria mellonella (L.) seventh instar larvae were investigated. The insects were reared from first instars on artificial diets containing 0.001, 0.01, 0.1 and 1.0 g EBIs per 100 g of diets. MDA content was significantly increased in the presence of esculetin. Low concentrations of esculetin led to increased GST activity. Dexamethasone at concentrations of 0.001 and 0.01% significantly resulted in increased hemolymph MDA content. However, high dietary dexamethasone concentrations caused significant decrease in MDA content while these concentrations resulted in increased GST activity by about three-fold. Dietary phenidone led to increased MDA content and GST activity in the larvae. The quantity of the 45 kDa protein fraction from total 16 protein detected was increased with increasing esculetin concentrations. An increase after phenidone and dexamethasone exposure was detected only at 0.001 and 0.01% concentration. Other main hemolymph proteins such as ApoLP-I and storage proteins were not altered in larvae reared on diets amended with the EBIs. We suggest that 45 kDa protein may be an oxidative stress-related protein in hemolymph of G. mellonella. This is the first demonstration that antioxidative responses to a EBI exposure are modulated by a physiological system that includes eicosanoid biosynthesis.