| STEFANIDESOVA, Katarina, Elena KOCIANOVA, Zina KOSTANOVA, Pavel KANKA, Danka NEMETHOVA and Eva SPITALSKA. Evidence of Anaplasma phagocytophilum and Rickettsia helvetica infection in free-ranging ungulates in central Slovakia. European Journal of Wildlife Research. Berlin Heidelberg: Springer, 2008, vol. 54, No 3, p. 519-524. ISSN 1612-4642. |
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@article{775204,
author = {Stefanidesova, Katarina and Kocianova, Elena and Kostanova, Zina and Kanka, Pavel and Nemethova, Danka and Spitalska, Eva},
article_location = {Berlin Heidelberg},
article_number = {3},
keywords = {Anaplasma spp.; Rickettsia spp.; Tick-borne diseases; Slovakia; Wildlife},
language = {eng},
issn = {1612-4642},
journal = {European Journal of Wildlife Research},
title = {Evidence of Anaplasma phagocytophilum and Rickettsia helvetica infection in free-ranging ungulates in central Slovakia},
volume = {54},
year = {2008}
}
TY - JOUR
ID - 775204
AU - Stefanidesova, Katarina - Kocianova, Elena - Kostanova, Zina - Kanka, Pavel - Nemethova, Danka - Spitalska, Eva
PY - 2008
TI - Evidence of Anaplasma phagocytophilum and Rickettsia helvetica infection in free-ranging ungulates in central Slovakia
JF - European Journal of Wildlife Research
VL - 54
IS - 3
SP - 519-524
EP - 519-524
PB - Springer
SN - 16124642
KW - Anaplasma spp.
KW - Rickettsia spp.
KW - Tick-borne diseases
KW - Slovakia
KW - Wildlife
N2 - The purpose of this study was to investigate the role of wild animals for Anaplasma phagocytophilum, other ehrlichiae/anaplasmae, Rickettsia helvetica and other rickettsiae and whether different genetic variants of A. phagocytophilum in central Slovakia exist. A total of 109 spleen samples from 49 red deer (Cervus elaphus), 30 roe deer (Capreolus capreolus), 28 wild boar (Sus scrofa) and two mouflon (Ovis musimon) were collected from June 2005 to December 2006. Polymerase chain reaction (PCR) amplification of the16S rRNA gene was used for detection of ehrlichiae/anaplasmae. A nested PCR targeting part (392 bp) of groESL gene was applied for the specific detection of A. phagocytophilum. Fragments of the gltA and ompA genes (381 bp and 632 bp, respectively) were amplified to detect rickettsiae, followed by sequencing. A. phagocytophilum and R. helvetica were detected in wild animals. The prevalence of A. phagocytophilum was 50.0% in roe deer and 53.1% in red deer. None of the 28 wild boar was PCR positive for ehrlichiae/anaplasmae. A. phagocytophilum was detected in one mouflon. R. helvetica was found in one roe deer. Our study suggests a role of cervids as a natural reservoir of A. phagocytophilum in Slovakia. However, the role of cervids and wild boars in the circulation of R. helvetica remains unknown. The analysis of sequence variation in the msp4 coding region of A. phagocytophilum showed the presence of different variants previously described in ruminants.
ER -
STEFANIDESOVA, Katarina, Elena KOCIANOVA, Zina KOSTANOVA, Pavel KANKA, Danka NEMETHOVA and Eva SPITALSKA. Evidence of Anaplasma phagocytophilum and Rickettsia helvetica infection in free-ranging ungulates in central Slovakia. \textit{European Journal of Wildlife Research}. Berlin Heidelberg: Springer, 2008, vol.~54, No~3, p.~519-524. ISSN~1612-4642.
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