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@inproceedings{775508, author = {Šulák, Ondřej and Delia, Monia and Kostlánová, Nikola and Imberty, Anne and Wimmerová, Michaela}, address = {Brno}, booktitle = {XII. Setkání biochemiků a molekulárních biologů}, keywords = {Lectin; Burkholderia cenocepacia; SPR; ITC}, language = {eng}, location = {Brno}, isbn = {978-80-210-4526-2}, pages = {53-54}, publisher = {Masarykova univerzita}, title = {CHARACTERISATION OF A NEW LECTIN BCLC FROM THE HUMAN OPPORTUNISTIC PATHOGEN BURKHOLDERIA CENOCEPACIA}, year = {2008} }
TY - JOUR ID - 775508 AU - Šulák, Ondřej - Delia, Monia - Kostlánová, Nikola - Imberty, Anne - Wimmerová, Michaela PY - 2008 TI - CHARACTERISATION OF A NEW LECTIN BCLC FROM THE HUMAN OPPORTUNISTIC PATHOGEN BURKHOLDERIA CENOCEPACIA PB - Masarykova univerzita CY - Brno SN - 9788021045262 KW - Lectin KW - Burkholderia cenocepacia KW - SPR KW - ITC N2 - Four genes coding proteins homologous to lectin PA-IIL from Pseudomonas aeruginasa have been found in the genome of B. cenocepacia. One of them, named BclC, is a 28 kDa protein which is able to recognize D-mannosylated carbohydrates. This protein also exhibits partial sequence homology with the protein CV-IIL from the Chromobacterium violaceum. BclC has been cloned and prepared in recombinant form. Sequence analysis showed two distinct domains in the protein, N-terminal part that does not have any sequence homolog in genomic and protein databases, and C-terminal part that codes for lectin domain. Both domains were also cloned separately to simplify further structure-function characterization. Detailed functional studies using surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) allowed to define binding properties (afinity, kinetics) and thermodynamic parameters. ER -
ŠULÁK, Ondřej, Monia DELIA, Nikola KOSTLÁNOVÁ, Anne IMBERTY and Michaela WIMMEROVÁ. CHARACTERISATION OF A NEW LECTIN BCLC FROM THE HUMAN OPPORTUNISTIC PATHOGEN BURKHOLDERIA CENOCEPACIA. In \textit{XII. Setkání biochemiků a molekulárních biologů}. Brno: Masarykova univerzita, 2008, p.~53-54. ISBN~978-80-210-4526-2.
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