Sensitivity analysis of RecA genotoxicity test

FLEGROVÁ, Zuzana, Tomáš BARTOŠ, Michal ŠKAREK and Pavel ČUPR. Sensitivity analysis of RecA genotoxicity test. Annals of Microbiology. 2008, vol. 58, No 1, p. 1-5. ISSN 1590-4261.

Basic information

Original name

Sensitivity analysis of RecA genotoxicity test

Name in Czech

viz. angl.

Authors

FLEGROVÁ, Zuzana (203 Czech Republic, guarantor), Tomáš BARTOŠ (203 Czech Republic), Michal ŠKAREK (203 Czech Republic) and Pavel ČUPR (203 Czech Republic)

Edition

Annals of Microbiology, 2008, 1590-4261

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.466

RIV identification code

RIV/00216224:14310/08:00026345

Organization unit

Faculty of Science

UT WoS

000254944600001

Keywords in English

genotoxicity RecA

Tags

genotoxicity RecA

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Abstract

V originále

RecA test is a short-term luminescent SOS genotoxicity test. The induction of the SOS system is detected through the activation of the reporter gene system - DNA damage SOS repair system. The test is based on the swift elevation of recA gene expression in response to DNA damage. In case of RecA test there is a specific recA::luxCDABE gene fusion. It is fusion of reporter gene and specific gene of SOS repair system. This gene fusion could detect genotoxicity efficiently. In this study Escherichia coli strains DPD2794, DPD2797, DPD1718 and DPD1710 were used for detection of genotoxicity. The genotoxicity was detected by monitoring of bioluminescent emission. For assessment of the sensitivity evaluation a group of chemicals and environmental samples were selected. The aim of this research was to decide whether this test has future applications in the screening evaluation of genotoxic potential and to compare sensitivity of the RecA test with various strains of Escherichia coli. Results indicate that the strain DPD2797 could be recommended for testing of environmental samples and also pure chemical substances, because this strain showed most sensitive responses to selected chemical standards and also to environmental samples.

In Czech

viz. angl.

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Links

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL) Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)