a 2008

Baseline levels of selected biochemical markers in Enchytraeus crypticus

ŠUTEKOVÁ, Eva, Klára HILSCHEROVÁ and Jakub HOFMAN

Basic information

Original name

Baseline levels of selected biochemical markers in Enchytraeus crypticus

Name in Czech

Bazální hladiny vybraných biochemických markerů v Enchytraeus crypticus

Authors

ŠUTEKOVÁ, Eva (203 Czech Republic), Klára HILSCHEROVÁ (203 Czech Republic) and Jakub HOFMAN (203 Czech Republic, guarantor)

Edition

Warsaw, Abstract Book, p. 98-98, 1 pp. 2008

Publisher

SETAC Europe

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10511 Environmental sciences

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/08:00024895

Organization unit

Faculty of Science

Keywords in English

bichemical markers; enchytraeids; baseline levels; soil

Tags

International impact, Reviewed
Změněno: 24/2/2010 20:40, prof. RNDr. Jakub Hofman, Ph.D.

Abstract

V originále

Enchytraeids are small soil annelids who have been frequently and successfully used as testing organisms to determine possible hazards of chemicals entering the soil or to evaluate contaminated soil quality. Standardized tests for Enchytraeus sp. target only mortality and reproduction as measurable endpoints. However, no-effect on survival and reproduction doesnt mean healthy animals. Oxidative stress caused by toxic substances or other environmental factors is one of the main toxicity mechanisms. Biochemical or cell processes alteration related to oxidative stress can serve as an early indication of more pronounced effects. Biochemical markers of oxidative stress and detoxification have never been investigated in enchytraeids, although their potential as sensitive parameters of chemical exposure might be expected. To enable use of these parametres in future studies with enchytraeids, baseline levels and variability have to be described first. In our study, glutathione - GSH, glutathione-S-transferase - GST, glutathione peroxidase - GPx, glutathione reductase - GR, and lipid perxidation - LP were measured in Enchytraeus crypticus samples from the age synchronized and non-synchronized populations cultured on agar or in the artificial soil. Dependence on sample size, and culture medium, as well as variability range and its sources were investigated.

In Czech

Není

Links

GP525/04/P159, research and development project
Name: Vliv nově rozpoznávaných prioritních perzistentních organických polutantů (POPs) na organismy v půdním ekosystému
Investor: Czech Science Foundation, Effects of newly recognized priority persistent organic pollutants (POPs) on organisms of soil ecosystem
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)