Detailed Information on Publication Record
2008
Identification of Staphylococcus spp. isolated from ixodid ticks.
ŠVEC, Pavel, Dana NOVÁKOVÁ, Ivo RUDOLF, Silvie ŠIKUTOVÁ, Jana MASAŘÍKOVÁ et. al.Basic information
Original name
Identification of Staphylococcus spp. isolated from ixodid ticks.
Name in Czech
Identifikace Staphylococcus spp. izolovaných z klíšťat
Authors
ŠVEC, Pavel (203 Czech Republic, guarantor), Dana NOVÁKOVÁ (203 Czech Republic), Ivo RUDOLF (203 Czech Republic), Silvie ŠIKUTOVÁ (203 Czech Republic), Jana MASAŘÍKOVÁ (203 Czech Republic), Zdeněk HUBÁLEK (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic)
Edition
Abstract Book, XII International Congress of Bacteriology and Applied Microbiology, 2008
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Turkey
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/08:00024266
Organization unit
Faculty of Science
Keywords in English
ixodid ticks; staphylococcus; identification; taxonomy
Tags
International impact, Reviewed
Změněno: 24/3/2010 12:10, doc. RNDr. Pavel Švec, Ph.D.
V originále
A group of eight Gram-positive, catalase positive cocci was isolated during analysis of cultivable microflora of ixodid ticks, namely Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna, vectors of many vertebrate pathogens. Analysed strains were isolated from whole-body homogenized surface-sterilized ticks inoculated on different kinds of media: Tryptone Soya Agar, Brain-Heart Infusion Agar and Columbia Blood Agar. Individual colonies were picked up, purified and preliminary identified by using rep-PCR fingerprinting with the primer (GTG)5. This method assigned three strains as Staphylococcus aureus subsp. aureus (DR11, HC37, HC48), two strains as Staphylococcus warneri (HC13, HC36) and the remaining strains represented Staphylococcus cohnii subsp. cohnii (DR8), Staphylococcus epidermidis (HC34) and Staphylococcus capitis subsp. capitis (HC31). These results were verified by automated ribotyping with EcoRI (RiboPrinter microbial characterization system) as well as whole-cell protein fingerprinting and confirmed the (GTG)5-PCR fingerprinting as a fast and reliable tool for identification of staphylococci. Biotyping using API ID 32 Staph kit misidentified S. cohnii subsp. cohnii (DR8) as Staphylococcus xylosus and S. warneri (HC13) as Staphylococcus haemolyticus. Two strains (DR11 and DR8) originated from Dermacentor reticulatus and the remaining strains were isolated from Haemaphysalis concinna. Interestingly, no staphylococci were isolated from Ixodes ricinus in our study. There is no evidence that staphylococci isolated in our study create integral part of tick internal microflora, obviously they are ingested incidentally during tick feeding on vertebrate host and they could survive in tick midgut. Financial support from project KJB600930613 is gratefully acknowledged.
In Czech
Práce popisuje identifikaci devíti kmenů rodu Staphylococcus izolovaných z klíšťat Ixodes ricinus, Dermacentor reticulatus a Haemaphysalis concinna. Všechny kmeny byly charakterizovány biotypizací, rep-PCR, ribotypizací a analýzou celkových buněčných proteinů a identifikovány jako Staphylococcus aureus subsp. aureus (kmeny DR11, HC37, HC48), Staphylococcus warneri (HC13, HC36), Staphylococcus cohnii subsp. cohnii (DR8), Staphylococcus epidermidis (HC34) a Staphylococcus capitis subsp. capitis (HC31).
Links
| KJB600930613, research and development project |
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