RUDOLF, Ivo, Silvie ŠIKUTOVÁ, Zdeněk HUBÁLEK, Jana MASAŘÍKOVÁ, Pavel ŠVEC, Dana NOVÁKOVÁ, Ivo SEDLÁČEK, Jan MENDEL and Ivo PAPOUŠEK. Diversity of cultivable microorganisms isolated from ixodid ticks. In Abstract Book, XII International Congress of Bacteriology and Applied Microbiology. 2008.
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Basic information
Original name Diversity of cultivable microorganisms isolated from ixodid ticks.
Name in Czech Diverzita kultivovatelných mikroorganismů izolovaných z klišťat
Authors RUDOLF, Ivo (203 Czech Republic, guarantor), Silvie ŠIKUTOVÁ (203 Czech Republic), Zdeněk HUBÁLEK (203 Czech Republic), Jana MASAŘÍKOVÁ (203 Czech Republic), Pavel ŠVEC (203 Czech Republic), Dana NOVÁKOVÁ (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic), Jan MENDEL (203 Czech Republic) and Ivo PAPOUŠEK (203 Czech Republic).
Edition Abstract Book, XII International Congress of Bacteriology and Applied Microbiology, 2008.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Turkey
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/08:00024267
Organization unit Faculty of Science
Keywords in English ixodid ticks; cultivable microflora; characterization
Tags Characterization, cultivable microflora, ixodid ticks
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Pavel Švec, Ph.D., učo 1098. Changed: 24/3/2010 12:08.
Abstract
Ixodid ticks (namely Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna) represent significant health risk for humans as well as many other vertebrate species as vectors of multiple pathogens. In addition, ticks harbour wealth of microorganisms which have not been associated with human illness so far. Polyphasic taxonomic approach was applied in the study to describe cultivable bacterial microflora of ixodid ticks. A total of 151 bacterial strains (67 strains from I. ricinus, 38 strains from D. reticulatus and 46 strains from H. concinna) were recovered during two-year examination from different developmental stages of field-collected ixodid ticks (larvae, nymphs, adults). Morphological, biochemical and molecular studies were performed. On the basis of partial 16S rDNA sequencing analysed strains belong most frequently to the genera Bacillus, Paenibacillus, Pseudomonas. According to preliminary data some strains might belong to new taxa. Moreover, many isolated microorganisms were able to grow at 37 C and displayed significant haemolytic activity on Columbia agar with sheep blood. These indirect criteria suggest possible pathogenic potential of certain isolates. Further studies are needed to elucidate whether strains could pose health threat for humans. In conclusion, ixodid ticks are still black boxes in wealth of microorganisms they can transmit and might serve as a source of potential emerging pathogens in future.
Abstract (in Czech)
Ixodid ticks (namely Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna) represent significant health risk for humans as well as many other vertebrate species as vectors of multiple pathogens. In addition, ticks harbour wealth of microorganisms which have not been associated with human illness so far. Polyphasic taxonomic approach was applied in the study to describe cultivable bacterial microflora of ixodid ticks. A total of 151 bacterial strains (67 strains from I. ricinus, 38 strains from D. reticulatus and 46 strains from H. concinna) were recovered during two-year examination from different developmental stages of field-collected ixodid ticks (larvae, nymphs, adults). Morphological, biochemical and molecular studies were performed. On the basis of partial 16S rDNA sequencing analysed strains belong most frequently to the genera Bacillus, Paenibacillus, Pseudomonas. According to preliminary data some strains might belong to new taxa. Moreover, many isolated microorganisms were able to grow at 37 C and displayed significant haemolytic activity on Columbia agar with sheep blood. These indirect criteria suggest possible pathogenic potential of certain isolates. Further studies are needed to elucidate whether strains could pose health threat for humans. In conclusion, ixodid ticks are still black boxes in wealth of microorganisms they can transmit and might serve as a source of potential emerging pathogens in future.
Links
KJB600930613, research and development projectName: Diverzita kultivovatelných mikroorganizmů v klíšťatech, vektorech patogenů obratlovců
Investor: Academy of Sciences of the Czech Republic, Diversity of cultivable microorganisms of ixodid ticks, recognized vectors of vertebrate pathogens
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