Detailed Information on Publication Record
2008
Identification of lactobacilli isolated from dental caries of children
ŠVEC, Pavel, Ivo SEDLÁČEK, Lenka ŽÁČKOVÁ and Martina KUKLETOVÁBasic information
Original name
Identification of lactobacilli isolated from dental caries of children
Name in Czech
Identifikace laktobacilů izolovaných ze zubního kazu dětí
Authors
ŠVEC, Pavel (203 Czech Republic, guarantor), Ivo SEDLÁČEK (203 Czech Republic), Lenka ŽÁČKOVÁ (203 Czech Republic) and Martina KUKLETOVÁ (203 Czech Republic)
Edition
9th Symposium on Lactic Acid Bacteria, Health, Evolution and Systems Biology, 2008
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/08:00026373
Organization unit
Faculty of Science
Keywords in English
Lactobacillus; dental caries; identification; taxonomy
Tags
International impact, Reviewed
Změněno: 24/3/2010 12:06, doc. RNDr. Pavel Švec, Ph.D.
V originále
The aim of this work was to analyse a group of 69 lactobacilli isolated from dental caries and root canals of the extracted teeth from early childhood caries (ECC) affected children up to age of six. Clinical samples obtained from individual patients were cultivated in a 5 % CO2 atmosphere on MRS agar and/or BHI agar supplemented by 10 % sheep blood. Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. Rep-PCR fingerprinting with the (GTG)5 primer was used for genotype characterization and identification of the isolates. Evaluation of obtained (GTG)5-PCR fingerprints assigned clearly 21 strains as Lactobacillus rhamnosus, 20 as Lactobacillus fermentum, 16 as Lactobacillus casei/paracasei, 7 as Lactobacillus salivarius, 4 as Lactobacillus plantarum and 1 as Lactobacillus gasseri.
In Czech
Cílem práce bylo taxonomicky identifikovat 69 kmenů laktobacilů izolovaných z zubního kazu dětí. S využitím biotypizace a rep-PCR s primerem (GTG)5 bylo identifikováno: 21 Lactobacillus rhamnosus, 20 Lactobacillus fermentum, 16 Lactobacillus casei/paracasei, 7 Lactobacillus salivarius, 4 Lactobacillus plantarum a 1 kmen Lactobacillus gasseri. Výsledky ukázaly použitou rep-PCR metodu jako vhodnou pro rychlou identifikaci laktobacilů vyskytujících se v zubním kazu.
Links
| MSM0021622416, plan (intention) |
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| 1M0528, research and development project |
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