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@proceedings{776877,
author = {Illichmanová, Adéla and Janů, Miroslava and Sedláček, Vojtěch and Kučera, Igor},
booktitle = {FEBS Journal Supplement 33rd FEBS Congress},
keywords = {nitrate reductase; electron donor; membrane permeability; specificity; viologen},
language = {eng},
title = {Re-examination of the rationale for the use of bipyridylium cation radicals as site-specific electron donors for respiratory nitrate reduction},
year = {2008}
}
TY - CONF
ID - 776877
AU - Illichmanová, Adéla - Janů, Miroslava - Sedláček, Vojtěch - Kučera, Igor
PY - 2008
TI - Re-examination of the rationale for the use of bipyridylium cation radicals as site-specific electron donors for respiratory nitrate reduction
KW - nitrate reductase
KW - electron donor
KW - membrane permeability
KW - specificity
KW - viologen
N2 - Paracoccus denitrificans express two distinct nitrate reductases that are associated with the respiratory chain: the membrane-bound Nar, and the periplasmic Nap. Their activity is usually measured using colored bipyridylium cation radicals methyl viologen (MV) or benzyl viologen (BV) as electron donors. It is generally believed that the more polar MV does not enter the cell and hence donates electrons exclusively to Nap, while oxidation of hydrophobic BV reflects the action of both enzymes. This issue was further examined by a kinetic analysis of nitrate reduction in the wild-type strain and in the Nar or Nap mutant strains. The results show that (i) MV is a quite effective donor for either enzyme, (ii) BV blocks the active transport of nitrate to the active site of Nar, and (iii) BV functions as an ionophore for nitrate at higher concentrations.
ER -
ILLICHMANOVÁ, Adéla, Miroslava JANŮ, Vojtěch SEDLÁČEK and Igor KUČERA. Re-examination of the rationale for the use of bipyridylium cation radicals as site-specific electron donors for respiratory nitrate reduction. In \textit{FEBS Journal Supplement 33rd FEBS Congress}. 2008. ISSN~1742-464X.
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