a 2008

Analysis of <i>A. thaliana</i> resistence to high light stress and reactive oxygen species formation using biophysical methods

BARTÁK, Miloš, Kristýna VEČEŘOVÁ, Jaroslav LANG, Peter VÁCZI, Hana CEMPÍRKOVÁ et. al.

Basic information

Original name

Analysis of <i>A. thaliana</i> resistence to high light stress and reactive oxygen species formation using biophysical methods

Name in Czech

Analýza odolnosti rostlin A. thalina proti účinku vysokých hodnot záření a tvorbě reaktivních forem kyslíku založená na biofyzikálních metodách

Authors

BARTÁK, Miloš (203 Czech Republic, guarantor, belonging to the institution), Kristýna VEČEŘOVÁ (203 Czech Republic, belonging to the institution), Jaroslav LANG (203 Czech Republic, belonging to the institution), Peter VÁCZI (703 Slovakia, belonging to the institution), Hana CEMPÍRKOVÁ (203 Czech Republic, belonging to the institution), Josef HÁJEK (203 Czech Republic, belonging to the institution), Břetislav BRZOBOHATÝ (203 Czech Republic) and Petr ILÍK (203 Czech Republic)

Edition

Book of Abstract, 5th Tri-National Arabidopsis Meeting, 2008

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/08:00024904

Organization unit

Faculty of Science

Keywords in English

photoinhibition; abiotic stress; light harvesting complexes; photosystem II; fluorescence

Tags

Změněno: 30/3/2012 16:36, prof. Ing. Miloš Barták, CSc.

Abstract

ORIG CZ

V originále

Using several biophysical and biochemical techniques, we studied regulatory mechanisms that protect photosynthetic apparatus, chloroplast and thylakoid membrane components in particular, against negative effects of high light-induced reactive oxygen species (ROS). In control (wt) and mutant plants of A. thaliana, the effect of excess light on primary photosynthetic processes was investigated by several chlorophyll fluorescence techniques, chlorophyll fluorescence imaging in particular. The aim was to quantify the effects of (1) reduced number of light harvesting complexes (LHCs), (2) reduced ability to quench excess light in photosystem II (3) application of electron transport blocker in photosystem II (DCMU) on sensitivity of Arabidopsis thaliana to photoinhibition. In our experiments, we used plants with (a) reduced number of LHCs, LHC proteins, main subunit of LHC II and total chlorophyll. Experimental plants exhibited either 20 % (Lhcb2-1) or 40 % (Lhcb2-12) reduction of the above components, and (b) npq mutants. After controlled phototinhibitory treatment (30 min, 2000 micromol m-2 s-1), Fv/Fm, Yield PS II, NPQ and qE, qI were measured in 60 min interval. Effect of photoinhibition was studied also by fast chlorophyll fluorescence induction kinetics (OJIPs) recorded before and after photoinhibitory treatment. The results indicated that reduced number of LHCs altered functioning of PS II, especially in quenching of excess light energy. However, capacity of photoprotective mechanisms in A. thaliana was sufficient to cope with a short-term stress in PS II.

In Czech

Pomocí metod indukované fluorescence chlorofylu in vivo byl studován vliv redukce světlosběrných komplexů ve fotosystému II rostlin A. thaliana na primární fotochemické procesy fotosyntézy.

Links

GA522/06/0979, research and development project
Name: Mechanismy odolnosti rostlin proti ROS indukovaným ve fotosyntetickém aparátu během fotoinhibičního stresu
Investor: Czech Science Foundation, Mechanism of plant resistance against ROS induced in photosynthetic apparatus by a photoinhibitory stress