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@inproceedings{777395,
author = {Hégrová, Blanka and Jedličková, Lenka and Havlová, Pavla and Havel, Josef},
address = {Ediční středisko Univerzity Pardubice},
booktitle = {VITAMINS 2008 Nutrition and Diagnostics},
edition = {1.},
keywords = {trichothecene mycotoxins; deoxynivalenol; barley; malt; gushing; HPLC; MALDI-TOF mass spectrometry},
language = {eng},
location = {Ediční středisko Univerzity Pardubice},
isbn = {978-80-7318-708-8},
pages = {86-87},
publisher = {Univerzita Tomáše Bati ve Zlíně},
title = {Determination of deoxynivalenol levels in barley, malt and intermediate products of malting process by high performance liquid chromatography and MALDI-TOF mass spectrometry},
year = {2008}
}
TY - JOUR
ID - 777395
AU - Hégrová, Blanka - Jedličková, Lenka - Havlová, Pavla - Havel, Josef
PY - 2008
TI - Determination of deoxynivalenol levels in barley, malt and intermediate products of malting process by high performance liquid chromatography and MALDI-TOF mass spectrometry
PB - Univerzita Tomáše Bati ve Zlíně
CY - Ediční středisko Univerzity Pardubice
SN - 9788073187088
KW - trichothecene mycotoxins
KW - deoxynivalenol
KW - barley
KW - malt
KW - gushing
KW - HPLC
KW - MALDI-TOF mass spectrometry
N2 - The deoxynivalenol (DON) is the most frequently detected mycotoxin in grain samples and their subsequent food products (beer); associated with primary gushing. The extracted samples were analysed using RP-HPLC - using mobile phase AcN/H2O (9:1); flow rate 0.6 ml.min-1; column temperature at 25 C; sample dosage 20 ul; detected at 220 nm. The LOD and the LOQ for DON was 0.16 and 0.53 ug.ml-1, respectively 127.8 and 426.2 ug.kg-1. Suitable matrix for MALDI-TOF MS analysis were found matrix of sodium azide and diamond suspension, quick and dirty spotting of sample and matrix solution 3:1. Positive reflector mode and PE with optimum mass set to 300 Da were used. Adducts of DON were observed. The LOD for DON was 0.6 ug.ml-1, resp. 480.0 ug.kg-1. In this study we found out that the levels of DON during malting process increased (maybe caused by DON releasing from enzymatic conjugates). The correlation between DON content and the level of gushing was negligible.
ER -
HÉGROVÁ, Blanka, Lenka JEDLIČKOVÁ, Pavla HAVLOVÁ and Josef HAVEL. Determination of deoxynivalenol levels in barley, malt and intermediate products of malting process by high performance liquid chromatography and MALDI-TOF mass spectrometry. In \textit{VITAMINS 2008 Nutrition and Diagnostics}. 1st ed. Ediční středisko Univerzity Pardubice: Univerzita Tomáše Bati ve Zlíně, 2008, p.~86-87. ISBN~978-80-7318-708-8.
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