Interaction of arsenate and cyanobacterial biomass (Microcystis sp.) Investigation of metabolites

ČMELÍK, Jiří, Marie KONEČNÁ and Jiří MACHÁT. Interaction of arsenate and cyanobacterial biomass (Microcystis sp.) Investigation of metabolites. In 35th ISEAC. 2008. ISBN 978-83-925754-4-3.

Basic information

• Original name: Interaction of arsenate and cyanobacterial biomass (Microcystis sp.) Investigation of metabolites
• Name in Czech: Interakce arseničnanu a sinicové biomasy (Microcystis sp.) Studium metabolitů
• Authors: ČMELÍK, Jiří (203 Czech Republic, belonging to the institution), Marie KONEČNÁ (203 Czech Republic, belonging to the institution) and Jiří MACHÁT (203 Czech Republic, guarantor, belonging to the institution).
• Edition: 35th ISEAC, 2008.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Poland
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/08:00026410
• Organization unit: Faculty of Science
• ISBN: 978-83-925754-4-3
• Keywords in English: arsenic; cyanobacteria; biotransformation; speciation
• Tags: arsenic, biotransformation, cyanobacteria, Speciation
• Tags: International impact

Abstract

Sorption and biotransformation of arsenate by water bloom forming cyanobacterial biomass (predominantly Microcystis sp.) has been studied in this work. Biomass coming from Brno dam reservoir was cultivated in river water enriched by arsenate for 6 days. Water and aqueous extracts of biomass were subjected to anion exchange chromatographic analysis (Hamilton PRP X-100 column, ammonium acetate mobile phase pH 6.0) with ICP-MS detection. Quantitative reduction of arsenate to arsenite was observed followed by biomethylation mainly to dimethylarsinate (DMA).

Abstract (in Czech)

V prácibyla studována sorpce arseničnanu sinicovou biomasou (Microcystis sp.). Biomasa z Brněnské přehradní nádrže byla kultivována ve vodě obohacené arseničnanem po dobu 6 dnů. Voda a vodné extrakty biomasy byly analyzovány iontovou chromatografií s ICP-MS detekcí. Byla pozorována kvantitativní redukce na arsenitan, následovaná biomethylací na dimethylarseničnan (DMA). Monomethylarseničnan (MMA), DMA a obě anorganické specie byly nalezeny a stanoveny v extraktu biomasy i ve vodě. V biomase byly nalezeny také neznámé specie As. Pro jejich identifikaci byly použity různé metody, např. pH-specifické generování hydridů poukázalo na možnou přítomnost MMA(III) v biomase, jako meziproduktu biomethylace.

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)