Characteristics of Staphylococcus aureus strains from 5
outbreaks of pemphigus neonatorum in the Czech Republic and
Slovakia

D 2008

Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia

PETRÁŠ, Petr, Ivana MACHOVÁ, Vladislava RŮŽIČKOVÁ and Roman PANTŮČEK

Basic information

Original name

Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia

Name in Czech

Charakterizace kmenů Staphylococcus aureus z 5 epidemických výskytů pemphigus neonatorum v České republice a na Slovensku

Authors

PETRÁŠ, Petr (203 Czech Republic, guarantor), Ivana MACHOVÁ (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic) and Roman PANTŮČEK (203 Czech Republic)

Edition

Cairns, Australia, 13th International Symposium on Staphylococci and Staphylococcal Infections, p. 94-95, 2 pp. 2008

Publisher

Australian Society for Antimicrobials

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Australia

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/08:00026432

Organization unit

Faculty of Science

Keywords in English

Staphylococcus aureus; exfoliative toxin; pemphigus neonatorum; molecular typing; pulsed field gel electrophoresis

Abstract

ORIG CZ

V originále

Objectives: To characterize S. aureus (SAU) strains producing exfoliative toxins A (ETA) and/or B (ETB) from 5 outbreaks of pemphigus neonatorum. Methods: As many as 366 SAU strains were referred to the National Reference Laboratory for Staphylococci, NIPH, Prague, from pemphigus neonatorum outbreaks in 4 Czech (A, B, C, and D) and 1 Slovak (E) hospitals in 2006 - 2008. The strains were tested by phenotypic (RPLA, phage-typing) and genotypic methods (PFGE; prophage carriage by multiplex PCR targeting prophages significant in lysogenic conversion of ETA production). Results: As many as 118 of the 366 isolates under study were found to produce one, or two of exfoliative toxins. Hospital A. Sixty-nine SAU strains originating from an outbreak persisting for more than 6 months included 12 producers of both ETA and ETB classified into phage type (PT) 3A/3C/55 from newborns and mothers and 8 isolates from health care professionals. Hospital B. Of 35 SAU isolates, 13 were ETA producers (PT 47/75) from newborns and 3 originated from nurses. Hospital C. Altogether 146 SAU strains originated from an extended outbreak (November 2006 through August 2007). Of 36 toxigenic strains, 11 produced ETA and 25 ETA+ETB. The exfoliatin producers showed an identical PFGE pattern. Twenty-nine ETA and/or ETB producers were from newborns (blister fluid, navel swabs, impetigo, paronychia), two originated from mothers (impetigo and nasal swab), and one from a nasal swab from a nurse. The detection of 4 ETA+ETB producers from the hospital environment was of high relevance. The ETA+ETB-positive strains isolated in Hospital C showed identical PFGE patterns as did one strain, ETA+ETB producer, from the Hospital D. Hospital D. Fifty-seven SAU strains from one outbreak of pemphigus neonatorum in 2008. Nineteen strains with hyperproduction of exfoliatin B originated from children and mothers (nasal swabs and impetigo), and one such strain was isolated from children's balm. Slovak Hospital E. A long-standing outbreak has been observed since 2003. Since November 2006, 44 SAU strains were referred to NRL. Fifteen strains were identical ETA producers (PT 47/75) from newborns' blisters and 1 such strain was a nasal isolate from a nurse. Genotyping revealed that the strains isolated in Slovak hospital E in 2003 and 2006 are of the same PFGE type. A highly similar PFGE profile was identified in the causative SAU strains in hospital B. Interestingly, all studied ET-positive strains were harboring the same types of prophages (group B and Fb-like). Conclusions: Genotyping was helpful in identifying the source of infection and controlling nosocomial outbreaks of pemhigus neonatorum in newborns.

In Czech

neuvedeno

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

PETRÁŠ, Petr, Ivana MACHOVÁ, Vladislava RŮŽIČKOVÁ and Roman PANTŮČEK. Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia. In 13th International Symposium on Staphylococci and Staphylococcal Infections. Cairns, Australia: Australian Society for Antimicrobials, 2008, p. 94-95.

@inproceedings{778561,
   author = {Petráš, Petr and Machová, Ivana and Růžičková, Vladislava and Pantůček, Roman},
   address = {Cairns, Australia},
   booktitle = {13th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {Staphylococcus aureus; exfoliative toxin; pemphigus neonatorum; molecular typing; pulsed field gel electrophoresis},
   language = {eng},
   location = {Cairns, Australia},
   pages = {94-95},
   publisher = {Australian Society for Antimicrobials},
   title = {Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia},
   url = {http://www.isssi2008.com/},
   year = {2008}
}

TY  - JOUR
ID  - 778561
AU  - Petráš, Petr - Machová, Ivana - Růžičková, Vladislava - Pantůček, Roman
PY  - 2008
TI  - Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia
PB  - Australian Society for Antimicrobials
CY  - Cairns, Australia
KW  - Staphylococcus aureus
KW  - exfoliative toxin
KW  - pemphigus neonatorum
KW  - molecular typing
KW  - pulsed field gel electrophoresis
UR  - http://www.isssi2008.com/
N2  - Objectives: To characterize S. aureus (SAU) strains producing exfoliative toxins A (ETA) and/or B (ETB) from 5 outbreaks of pemphigus neonatorum. Methods: As many as 366 SAU strains were referred to the National Reference Laboratory for Staphylococci, NIPH, Prague, from pemphigus neonatorum outbreaks in 4 Czech (A, B, C, and D) and 1 Slovak (E) hospitals in 2006 - 2008. The strains were tested by phenotypic (RPLA, phage-typing) and genotypic methods (PFGE; prophage carriage by multiplex PCR targeting prophages significant in lysogenic conversion of ETA production). Results: As many as 118 of the 366 isolates under study were found to produce one, or two of exfoliative toxins. Hospital A. Sixty-nine SAU strains originating from an outbreak persisting for more than 6 months included 12 producers of both ETA and ETB classified into phage type (PT) 3A/3C/55 from newborns and mothers and 8 isolates from health care professionals. Hospital B. Of 35 SAU isolates, 13 were ETA producers (PT 47/75) from newborns and 3 originated from nurses. Hospital C. Altogether 146 SAU strains originated from an extended outbreak (November 2006 through August 2007). Of 36 toxigenic strains, 11 produced ETA and 25 ETA+ETB. The exfoliatin producers showed an identical PFGE pattern. Twenty-nine ETA and/or ETB producers were from newborns (blister fluid, navel swabs, impetigo, paronychia), two originated from mothers (impetigo and nasal swab), and one from a nasal swab from a nurse. The detection of 4 ETA+ETB producers from the hospital environment was of high relevance. The ETA+ETB-positive strains isolated in Hospital C showed identical PFGE patterns as did one strain, ETA+ETB producer, from the Hospital D. Hospital D. Fifty-seven SAU strains from one outbreak of pemphigus neonatorum in 2008. Nineteen strains with hyperproduction of exfoliatin B originated from children and mothers (nasal swabs and impetigo), and one such strain was isolated from children's balm. Slovak Hospital E. A long-standing outbreak has been observed since 2003. Since November 2006, 44 SAU strains were referred to NRL. Fifteen strains were identical ETA producers (PT 47/75) from newborns' blisters and 1 such strain was a nasal isolate from a nurse. Genotyping revealed that the strains isolated in Slovak hospital E in 2003 and 2006 are of the same PFGE type. A highly similar PFGE profile was identified in the causative SAU strains in hospital B. Interestingly, all studied ET-positive strains were harboring the same types of prophages (group B and Fb-like). Conclusions: Genotyping was helpful in identifying the source of infection and controlling nosocomial outbreaks of pemhigus neonatorum in newborns.
ER  -

PETRÁŠ, Petr, Ivana MACHOVÁ, Vladislava RŮŽIČKOVÁ and Roman PANTŮČEK. Characteristics of $<$I$>$Staphylococcus aureus$<$/I$>$ strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia. In \textit{13th International Symposium on Staphylococci and Staphylococcal Infections}. Cairns, Australia: Australian Society for Antimicrobials, 2008, p.~94-95.

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