Significant overexpression of Hsp110 gene during colorectal
cancer progression.

J 2009

Significant overexpression of Hsp110 gene during colorectal cancer progression.

SLABÝ, Ondřej, Kateřina SOBKOVÁ, Marek SVOBODA, Ingrid GARAJOVÁ, Pavel FABIAN et. al.

Basic information

Original name

Significant overexpression of Hsp110 gene during colorectal cancer progression.

Name in Czech

Signifikantní nárůst exprese genu Hsp110 v průběhu progrese kolorektálního karcinomu.

Authors

Edition

Oncology Reports, Athens, Greece, Spandidos Publications Ltd. 2009, 1021-335X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Greece

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.588

Organization unit

Faculty of Medicine

UT WoS

000265274500014

Keywords in English

colorectal cancer; staging; lymph nodes; progression; gene expression profiles; Hsp110

Abstract

ORIG CZ

V originále

Colorectal cancer (CRC) is one of the most frequent malignant disease in the world. Metastatic spread of the cancer to the lymph nodes is crutial factor for progression and therapeutic management of the disease. We suppose that analysis of gene expression profiles of CRC patiens by low-density cancer-focused oligonucleotide microarrays enable us to identify new predictive markers of the extend of disease and can lead to a better understanding of CRC progression. Relative expression levels of 440 genes known to be involved in cancer biology were obtained by low-density oligonucleotide microarrays from 20 tumor samples. Statistical analysis of gene expression data identified 3 genes (HSP110, HYOU1, TCTP) significantly up-regulated in primary tumors of patients who developed lymph nodes metastasis. We have shown, for the first time, that up-regulation HSP110 and HYOU1 expression is associated with lymph nodes involvement in CRC. We validated the differences in HSP110 expression in an independent group of 30 patients of all clinical stages by Real-Time PCR method. We identified significant up-regulation of HSP110 expression in colorectal tumors compared to adjacent non-tumoral tissue (p<0,0003). For the first time, we observed significant differences of HSP110 gene expression between metastatic and localized disease (p=0,031) and negative trend of HSP110 gene expression and overall survival of CRC patients. We suggest that HSP110 gene is promising molecular predictor in CRC.

In Czech

Siginfikantní nárůst exprese genu Hsp110 v průběhu progrese kolorektálního karcinomu.

Links

NR9076, research and development project

Name: Genomické profilování v predikci odpovědi na chemoradioterapii u pacientů s lokálně pokročilým karcinomem konečníku

Citovat

SLABÝ, Ondřej, Kateřina SOBKOVÁ, Marek SVOBODA, Ingrid GARAJOVÁ, Pavel FABIAN, Roman HRSTKA, Rudolf NENUTIL, Milana ŠACHLOVÁ, Ilona KOCÁKOVÁ, Jaroslav MICHÁLEK, Tamara ŠMERDOVÁ, Dana KNOFLÍČKOVÁ and Rostislav VYZULA. Significant overexpression of Hsp110 gene during colorectal cancer progression. Oncology Reports. Athens, Greece: Spandidos Publications Ltd., 2009, vol. 2009, No 21, p. 1235-1241. ISSN 1021-335X.

@article{780215,
   author = {Slabý, Ondřej and Sobková, Kateřina and Svoboda, Marek and Garajová, Ingrid and Fabian, Pavel and Hrstka, Roman and Nenutil, Rudolf and Šachlová, Milana and Kocáková, Ilona and Michálek, Jaroslav and Šmerdová, Tamara and Knoflíčková, Dana and Vyzula, Rostislav},
   article_location = {Athens, Greece},
   article_number = {21},
   keywords = {colorectal cancer; staging; lymph nodes; progression; gene expression profiles; Hsp110},
   language = {eng},
   issn = {1021-335X},
   journal = {Oncology Reports},
   title = {Significant overexpression of Hsp110 gene during colorectal cancer progression.},
   url = {http://www.spandidos-publications.com/or/},
   volume = {2009},
   year = {2009}
}

TY  - JOUR
ID  - 780215
AU  - Slabý, Ondřej - Sobková, Kateřina - Svoboda, Marek - Garajová, Ingrid - Fabian, Pavel - Hrstka, Roman - Nenutil, Rudolf - Šachlová, Milana - Kocáková, Ilona - Michálek, Jaroslav - Šmerdová, Tamara - Knoflíčková, Dana - Vyzula, Rostislav
PY  - 2009
TI  - Significant overexpression of Hsp110 gene during colorectal cancer progression.
JF  - Oncology Reports
VL  - 2009
IS  - 21
SP  - 1235-1241
EP  - 1235-1241
PB  - Spandidos Publications Ltd.
SN  - 1021335X
KW  - colorectal cancer
KW  - staging
KW  - lymph nodes
KW  - progression
KW  - gene expression profiles
KW  - Hsp110
UR  - http://www.spandidos-publications.com/or/
N2  - Colorectal cancer (CRC) is one of the most frequent malignant disease in the world. Metastatic spread of the cancer to the lymph nodes is crutial factor for progression and therapeutic management of the disease. We suppose that analysis of gene expression profiles of CRC patiens by low-density cancer-focused oligonucleotide microarrays enable us to identify new predictive markers of the extend of disease and can lead to a better understanding of CRC progression. Relative expression levels of 440 genes known to be involved in cancer biology were obtained by low-density oligonucleotide microarrays from 20 tumor samples. Statistical analysis of gene expression data identified 3 genes (HSP110, HYOU1, TCTP) significantly up-regulated in primary tumors of patients who developed lymph nodes metastasis. We have shown, for the first time, that up-regulation HSP110 and HYOU1 expression is associated with lymph nodes involvement in CRC. We validated the differences in HSP110 expression in an independent group of 30 patients of all clinical stages by Real-Time PCR method. We identified significant up-regulation of HSP110 expression in colorectal tumors compared to adjacent non-tumoral tissue (p<0,0003). For the first time, we observed significant differences of HSP110 gene expression between metastatic and localized disease (p=0,031) and negative trend of HSP110 gene expression and overall survival of CRC patients. We suggest that HSP110 gene is promising molecular predictor in CRC.
ER  -

SLABÝ, Ondřej, Kateřina SOBKOVÁ, Marek SVOBODA, Ingrid GARAJOVÁ, Pavel FABIAN, Roman HRSTKA, Rudolf NENUTIL, Milana ŠACHLOVÁ, Ilona KOCÁKOVÁ, Jaroslav MICHÁLEK, Tamara ŠMERDOVÁ, Dana KNOFLÍČKOVÁ and Rostislav VYZULA. Significant overexpression of Hsp110 gene during colorectal cancer progression. \textit{Oncology Reports}. Athens, Greece: Spandidos Publications Ltd., 2009, vol.~2009, No~21, p.~1235-1241. ISSN~1021-335X.

Detailed Information on Publication Record