J 2008

DNA ploidy level variability of some fescues (Festuca subg. Festuca, Poaceae) from Central and Southern Europe measured in fresh plants and herbarium specimens

ŠMARDA, Petr

Basic information

Original name

DNA ploidy level variability of some fescues (Festuca subg. Festuca, Poaceae) from Central and Southern Europe measured in fresh plants and herbarium specimens

Name in Czech

Variabilita v DNA ploidních úrovních některých kostřav (Festuca subg. Festuca, Poaceae) ze střední a jižní evropy měřené u živých rostlin a herbářových položek

Authors

ŠMARDA, Petr (203 Czech Republic, guarantor)

Edition

Biologia, Bratislava, Slovak Academy of Sciences, 2008, 0006-3088

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.406

RIV identification code

RIV/00216224:14310/08:00026533

Organization unit

Faculty of Science

UT WoS

000256079800012

Keywords in English

Fescue; flow cytometry; hybridisation; mixed ploidy populations; polyploidy; Alps; Carpathians

Tags

International impact, Reviewed
Změněno: 2/2/2010 08:22, Mgr. Petr Šmarda, Ph.D.

Abstract

V originále

Using flow cytometry in fresh plants and herbarium vouchers, DNA ploidy levels for 411 individuals of 44 taxa of the genus Festuca, including 4 natural hybrids, originating from 237 sites in Austria, Bulgaria, Croatia, Czech Republic, Estonia, Germany, Hungary, Italy, Poland, Romania, Slovakia, Slovenia, and Switzerland were estimated. The following taxa and DNA ploidy levels are reported: F. airoides (2n = 2x), F. alpestris (2n = 2x), F. alpina s.l. (2n = 2x), F. amethystina subsp. amethystina (2n = 4x), F. bosniaca subsp. bosniaca (2n = 2x), F. brevipila (2n = 6x), F. bucegiensis (2n = 2x), F. carnuntina (2n = 6x), F. csikhegyensis (2n = 4x), F. csikhegyensis x F. eggleri (2n = 4x), F. dalmatica (2n = 4x), F. duvalii (2n = 4x), F. eggleri (2n = 2x, 4x), F. filiformis (2n = 2x), F. glauca (2n = 6x), F. heterophylla (2n = 4x), F. inops (2n = 2x), F. laevigata (2n = 8x), F. laxa (2n = 4x), F. lemanii (2n = 6x), F. norica (2n = 2x), F. ovina subsp. ovina (2n = 2x), F. ovina subsp. guesfalica (2n = 4x), F. ovina x F. pallens (2n = 4x), F. pallens (2n = 2x, 3x), F. pallens x F. pseudodalmatica (2n = 3x, 4x), F. pirinica (2n = 2x), F. polesica (2n = 2x), F. psammophila subsp. dominii (2n = 2x), F. pseudodalmatica (2n = 4x), F. pseudovina (2n = 2x), F. quadriflora (2n = 4x), F. rupicola (2n = 6x), F. rupicola x F. vaginata (2n = 3x, 4x), F. saxatilis (2n = 6x), F. stricta subsp. bauzanina (2n = 8x), F. supina (2n = 4x), F. tatrae (2n = 2x), F. valesiaca (2n = 2x), F. versicolor subsp. pallidula (2n = 2x), F. versicolor subsp. versicolor (2n = 2x), F. violacea subsp. puccinellii (2n = 2x), F. wagneri (2n = 4x), F. xanthina (2n = 2x). In F. pallens, up to 12-year-old herbarium specimens were proved to be suitable for DNA ploidy level measurements with flow cytometry. DNA ploidy levels of F. bucegiensis, F. bosniaca, and F. versicolor subsp. pallidula are reported here for the first time. The taxonomy of some polyploid complexes and several records of mixed ploidy level populations are briefly discussed. Festuca pseudodalmatica and its hybrid F. x krizoviensis were first recognised as native to the Czech Republic, and F. brevipila as native to Hungary. Also some new records of F. filiformis, F. brevipila, and F. wagneri from Slovakia are reported.

In Czech

Using flow cytometry in fresh plants and herbarium vouchers, DNA ploidy levels for 411 individuals of 44 taxa of the genus Festuca, including 4 natural hybrids, originating from 237 sites in Austria, Bulgaria, Croatia, Czech Republic, Estonia, Germany, Hungary, Italy, Poland, Romania, Slovakia, Slovenia, and Switzerland were estimated. The following taxa and DNA ploidy levels are reported: F. airoides (2n = 2x), F. alpestris (2n = 2x), F. alpina s.l. (2n = 2x), F. amethystina subsp. amethystina (2n = 4x), F. bosniaca subsp. bosniaca (2n = 2x), F. brevipila (2n = 6x), F. bucegiensis (2n = 2x), F. carnuntina (2n = 6x), F. csikhegyensis (2n = 4x), F. csikhegyensis x F. eggleri (2n = 4x), F. dalmatica (2n = 4x), F. duvalii (2n = 4x), F. eggleri (2n = 2x, 4x), F. filiformis (2n = 2x), F. glauca (2n = 6x), F. heterophylla (2n = 4x), F. inops (2n = 2x), F. laevigata (2n = 8x), F. laxa (2n = 4x), F. lemanii (2n = 6x), F. norica (2n = 2x), F. ovina subsp. ovina (2n = 2x), F. ovina subsp. guesfalica (2n = 4x), F. ovina x F. pallens (2n = 4x), F. pallens (2n = 2x, 3x), F. pallens x F. pseudodalmatica (2n = 3x, 4x), F. pirinica (2n = 2x), F. polesica (2n = 2x), F. psammophila subsp. dominii (2n = 2x), F. pseudodalmatica (2n = 4x), F. pseudovina (2n = 2x), F. quadriflora (2n = 4x), F. rupicola (2n = 6x), F. rupicola x F. vaginata (2n = 3x, 4x), F. saxatilis (2n = 6x), F. stricta subsp. bauzanina (2n = 8x), F. supina (2n = 4x), F. tatrae (2n = 2x), F. valesiaca (2n = 2x), F. versicolor subsp. pallidula (2n = 2x), F. versicolor subsp. versicolor (2n = 2x), F. violacea subsp. puccinellii (2n = 2x), F. wagneri (2n = 4x), F. xanthina (2n = 2x). In F. pallens, up to 12-year-old herbarium specimens were proved to be suitable for DNA ploidy level measurements with flow cytometry. DNA ploidy levels of F. bucegiensis, F. bosniaca, and F. versicolor subsp. pallidula are reported here for the first time. The taxonomy of some polyploid complexes and several records of mixed ploidy level populations are briefly discussed. Festuca pseudodalmatica and its hybrid F. x krizoviensis were first recognised as native to the Czech Republic, and F. brevipila as native to Hungary. Also some new records of F. filiformis, F. brevipila, and F. wagneri from Slovakia are reported.

Links

LC06073, research and development project
Name: Centrum pro výzkum biodiverzity
Investor: Ministry of Education, Youth and Sports of the CR, Biodiversity Research Center
MSM0021622416, plan (intention)
Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time