Gel electrophoresis of metalloproteins and laser ablation with inductively coupled plasma mass spectrometry

HAVLIŠ, Jan, Hana OVADOVÁ, Tomáš VACULOVIČ and Viktor KANICKÝ. Gel electrophoresis of metalloproteins and laser ablation with inductively coupled plasma mass spectrometry. In 9th European on Workshop in Elemental and Isotopic Analysis. 2008.

Basic information

• Original name: Gel electrophoresis of metalloproteins and laser ablation with inductively coupled plasma mass spectrometry
• Name in Czech: Gelová elektroforéza metaloproteinů a laserová ablace s induktivně vázaným plazmatem v kombinaci s hmotnostní spkterometrií
• Authors: HAVLIŠ, Jan (203 Czech Republic, guarantor), Hana OVADOVÁ (203 Czech Republic), Tomáš VACULOVIČ (203 Czech Republic) and Viktor KANICKÝ (203 Czech Republic).
• Edition: 9th European on Workshop in Elemental and Isotopic Analysis, 2008.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/08:00029111
• Organization unit: Faculty of Science
• Keywords in English: metalloprotein;speciation;gel electrophoresis;laser ablation;ICP MS
• Tags: gel electrophoresis, ICP MS, laser ablation, metalloprotein, Speciation
• Tags: International impact

Abstract

Optimal laser power density was found between 0.2 GW.cm-2 to 1.5 GW.cm-2. It is characteristic by the lowest RSD and the highest ICP MS signal. ICP MS signal is higher after ablation of gel, which was dried in the drier overnight than after ablation of gel, which was dried between cellophane foil. Background of 59Co signal is higher in the cellophane foil dried gel, but this gel showed easier handling in comparison with gel dried in drier. S/N ratio of LA-ICP-MS gel dried in the gel drier (6.41) is higher than in case of gel dried between cellophane foils (1.91).

Abstract (in Czech)

Nalezná optimální hustota zářivého výkonu laseru mezi 0.2 GW.cm-2 to 1.5 GW.cm-2; má nejnižší RSD a nejvyšší intezitu signálu v ICP-MS. ICP MS signál je vyšší pro gel sušený přes noc v sušicce gelů než mezi celofánovými fóliemi. Gelmsusšený mezi fóliemi má vyšší pozadí, ale snáze se s ním manipuluje. Poměr S/N u LA-ICP-MS gelu sušeného v sušičce (6.41) je vyšší než u gelu sušeného mezi celofánovými fóliemi (1.91).

Links

• GA203/07/1391, research and development project: Name: Vývoj nových metod pro charakterizaci metaloproteinů

Investor: Czech Science Foundation, Development of new methods for metalloprotein characterisation

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations