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@inproceedings{785829,
author = {Kostlánová, Nikola and Svobodová, Dana and Wimmerová, Michaela},
address = {Oxford (UK)},
booktitle = {FEBS Journal Volume 275 Supplement 1},
edition = {2008},
keywords = {tuberculosisů; mycobacterial glycosyltransferases; heterologic expression},
language = {eng},
location = {Oxford (UK)},
pages = {169-169},
publisher = {Blackwell Publishing Ltd.},
title = {USING DIFFERENT APPROACHES IN PREPARATION OF PUTATIVE MYCOBACTERIAL GLYCOSYLTRANSFERASES RV3782 AND RV0225 FOR CRYSTALLOGRAPHIC STUDIES},
year = {2008}
}
TY - JOUR
ID - 785829
AU - Kostlánová, Nikola - Svobodová, Dana - Wimmerová, Michaela
PY - 2008
TI - USING DIFFERENT APPROACHES IN PREPARATION OF PUTATIVE MYCOBACTERIAL GLYCOSYLTRANSFERASES RV3782 AND RV0225 FOR CRYSTALLOGRAPHIC STUDIES
PB - Blackwell Publishing Ltd.
CY - Oxford (UK)
KW - tuberculosisů
KW - mycobacterial glycosyltransferases
KW - heterologic expression
N2 - With using bioinformatics tools, several therapeutically famous glycosyltranferases (GTs) have been identified in Mycobacterium tuberculosis genom and genes rv3782 and rv0225 were amplified and cloned into the different expression vectors in order to provide their expression, purification and characterization. The task was complicated by the nature of glycosyltransferases, which are often unstable, hydrophobic and hard to isolate and purify. Therefore, the performance of various expression systems was explored in order to identify the best experimental routes.
ER -
KOSTLÁNOVÁ, Nikola, Dana SVOBODOVÁ and Michaela WIMMEROVÁ. USING DIFFERENT APPROACHES IN PREPARATION OF PUTATIVE MYCOBACTERIAL GLYCOSYLTRANSFERASES RV3782 AND RV0225 FOR CRYSTALLOGRAPHIC STUDIES. In \textit{FEBS Journal Volume 275 Supplement 1}. 2008th ed. Oxford (UK): Blackwell Publishing Ltd., 2008, p.~169. ISSN~1742-464X.
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