| BLANCHARD, Bertrand, Alessandra NURISSO, Emilie HOLLWILLE, Cecile TETAUD, Joele WIELLS, Martina POKORNÁ, Michaela WIMMEROVÁ, Annabelle VARROTTE and Anne IMBERTY. Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I. Journal of Molecular Biology. Amsterdam: Elsevier, 2008, vol. 383, No 4, p. 837-853. ISSN 0022-2836. |
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@article{787074,
author = {Blanchard, Bertrand and Nurisso, Alessandra and Hollwille, Emilie and Tetaud, Cecile and Wiells, Joele and Pokorná, Martina and Wimmerová, Michaela and Varrotte, Annabelle and Imberty, Anne},
article_location = {Amsterdam},
article_number = {4},
keywords = {pseudomonas aeruginosa; lectin; molecular modeling; structure;glycosphinglipid; oligosaccharides},
language = {eng},
issn = {0022-2836},
journal = {Journal of Molecular Biology},
title = {Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I},
volume = {383},
year = {2008}
}
TY - JOUR
ID - 787074
AU - Blanchard, Bertrand - Nurisso, Alessandra - Hollwille, Emilie - Tetaud, Cecile - Wiells, Joele - Pokorná, Martina - Wimmerová, Michaela - Varrotte, Annabelle - Imberty, Anne
PY - 2008
TI - Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I
JF - Journal of Molecular Biology
VL - 383
IS - 4
SP - 837-853
EP - 837-853
PB - Elsevier
SN - 00222836
KW - pseudomonas aeruginosa
KW - lectin
KW - molecular modeling
KW - structure;glycosphinglipid
KW - oligosaccharides
N2 - The opportunistic pathogen Pseudomonas aeruginosa contains several carbohydrate-binding proteins, among which is the P. aeruginosa lectin I (PA-IL), which displays affinity for a-galactosylated glycans. Glycan arrays were screened and demonstrated stronger binding of PA-IL toward aGal1-4bGal-terminating structures and weaker binding to aGal1-3bGal ones in order to determine which human glycoconjugates could play a role in the carbohydrate-mediated adhesion of the bacteria. This was confirmed in vivo by testing the binding of the lectin to Burkitt lymphoma cells that present large amounts of globotriaosylceramide antigen Gb3/CD77/Pk. Trisaccharide moieties of Gb3 and isoglobotriaosylceramide were tested by titration microcalorimetry, and both displayed similar affinity to PA-IL in solution.
ER -
BLANCHARD, Bertrand, Alessandra NURISSO, Emilie HOLLWILLE, Cecile TETAUD, Joele WIELLS, Martina POKORNÁ, Michaela WIMMEROVÁ, Annabelle VARROTTE and Anne IMBERTY. Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I. \textit{Journal of Molecular Biology}. Amsterdam: Elsevier, 2008, vol.~383, No~4, p.~837-853. ISSN~0022-2836.
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