Detailed Information on Publication Record
2008
Transduction of resistance plasmids by bacteriophages induced from methicillin-resistans Staphylococcus aureus strains
KUNTOVÁ, Lucie, Ivana NEPEJCHALOVÁ, Marian VARGA, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ et. al.Basic information
Original name
Transduction of resistance plasmids by bacteriophages induced from methicillin-resistans Staphylococcus aureus strains
Name in Czech
Transdukce plazmidů bakteriofágy indukovanými z meticilin rezistentních kmenů Staphylococcus aureus
Authors
KUNTOVÁ, Lucie (203 Czech Republic), Ivana NEPEJCHALOVÁ (203 Czech Republic), Marian VARGA (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic, guarantor)
Edition
Abstract book. Würzburg, Germany, Pathophysiology of Staphylococci, p. 49-49, 2008
Publisher
Transregional Collaborative Research Center 34
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/08:00026646
Organization unit
Faculty of Science
Keywords in English
Staphylococcus aureus; antimicrobial resistance; horizontal gene transfer; bacteriophages
Tags
International impact
Změněno: 31/10/2008 14:52, prof. RNDr. Roman Pantůček, Ph.D.
V originále
Our work was focused on generalized transduction of resistance plasmids in MRSA strains. In a set of 66 clinical MRSA strains the plasmid content and lysogenicity were estimated. Lysogenic strains harboring tetracycline and penicillinase plasmids were chosen as the donor strains. The transduction were performed with phage lysates obtained by UV-irradiation of the donor strains. The prophage-less strain 8325-4, restriction-deficient strains SA113 and SA1039, and some clinical MRSA strains were used as recipients. Both penicillinase (30.2 kb) and tetracycline (4.4 kb) resistance plasmids were independently transferred into recipient strains SA113 and 8325-4. The transductants were characterized in detail in respect to transferred plasmids, antibiotic resistance phenotype and lysogenicity with transducing phages. The transducing phage induced from an MRSA strain differs from the well defined transducing phages 11, 80alpha and 53 in all tested characteristics, i.e. in integrase type, in the sequence of portal gene and in restriction endonuclease patterns (HindIII and EcoRI). It is suggested that prophages with transducing ability are responsible for dissemination of resistance determinants in populations of MRSA.
In Czech
Our work was focused on generalized transduction of resistance plasmids in MRSA strains. In a set of 66 clinical MRSA strains the plasmid content and lysogenicity were estimated. Lysogenic strains harboring tetracycline and penicillinase plasmids were chosen as the donor strains. The transduction were performed with phage lysates obtained by UV-irradiation of the donor strains. The prophage-less strain 8325-4, restriction-deficient strains SA113 and SA1039, and some clinical MRSA strains were used as recipients. Both penicillinase (30.2 kb) and tetracycline (4.4 kb) resistance plasmids were independently transferred into recipient strains SA113 and 8325-4. The transductants were characterized in detail in respect to transferred plasmids, antibiotic resistance phenotype and lysogenicity with transducing phages. The transducing phage induced from an MRSA strain differs from the well defined transducing phages 11, 80alpha and 53 in all tested characteristics, i.e. in integrase type, in the sequence of portal gene and in restriction endonuclease patterns (HindIII and EcoRI). It is suggested that prophages with transducing ability are responsible for dissemination of resistance determinants in populations of MRSA.
Links
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