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@inproceedings{788637,
author = {Kuntová, Lucie and Nepejchalová, Ivana and Varga, Marian and Pantůček, Roman and Růžičková, Vladislava and Doškař, Jiří},
address = {Würzburg, Germany},
booktitle = {Pathophysiology of Staphylococci},
edition = {Abstract book},
keywords = {Staphylococcus aureus; antimicrobial resistance; horizontal gene transfer; bacteriophages},
language = {eng},
location = {Würzburg, Germany},
pages = {49-49},
publisher = {Transregional Collaborative Research Center 34},
title = {Transduction of resistance plasmids by bacteriophages induced from methicillin-resistans Staphylococcus aureus strains},
url = {http://www.pathostaph.de/},
year = {2008}
}
TY - JOUR
ID - 788637
AU - Kuntová, Lucie - Nepejchalová, Ivana - Varga, Marian - Pantůček, Roman - Růžičková, Vladislava - Doškař, Jiří
PY - 2008
TI - Transduction of resistance plasmids by bacteriophages induced from methicillin-resistans Staphylococcus aureus strains
PB - Transregional Collaborative Research Center 34
CY - Würzburg, Germany
KW - Staphylococcus aureus
KW - antimicrobial resistance
KW - horizontal gene transfer
KW - bacteriophages
UR - http://www.pathostaph.de/
N2 - Our work was focused on generalized transduction of resistance plasmids in MRSA strains. In a set of 66 clinical MRSA strains the plasmid content and lysogenicity were estimated. Lysogenic strains harboring tetracycline and penicillinase plasmids were chosen as the donor strains. The transduction were performed with phage lysates obtained by UV-irradiation of the donor strains. The prophage-less strain 8325-4, restriction-deficient strains SA113 and SA1039, and some clinical MRSA strains were used as recipients. Both penicillinase (30.2 kb) and tetracycline (4.4 kb) resistance plasmids were independently transferred into recipient strains SA113 and 8325-4. The transductants were characterized in detail in respect to transferred plasmids, antibiotic resistance phenotype and lysogenicity with transducing phages. The transducing phage induced from an MRSA strain differs from the well defined transducing phages 11, 80alpha and 53 in all tested characteristics, i.e. in integrase type, in the sequence of portal gene and in restriction endonuclease patterns (HindIII and EcoRI). It is suggested that prophages with transducing ability are responsible for dissemination of resistance determinants in populations of MRSA.
ER -
KUNTOVÁ, Lucie, Ivana NEPEJCHALOVÁ, Marian VARGA, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jiří DOŠKAŘ. Transduction of resistance plasmids by bacteriophages induced from methicillin-resistans Staphylococcus aureus strains. In \textit{Pathophysiology of Staphylococci}. Abstract book. Würzburg, Germany: Transregional Collaborative Research Center 34, 2008, p.~49.
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