Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic

RŮŽIČKOVÁ, Vladislava, Pavla HOLOCHOVÁ, Renata KARPÍŠKOVÁ, Roman PANTŮČEK, Markéta POSPÍŠILOVÁ a Jiří DOŠKAŘ. Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic. In Pathophysiology of Staphylococci. Abstract book. Würzburg, Germany: Transregional Collaborative Research Center 34. s. 77. 2008.

Základní údaje

• Originální název: Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic
• Název česky: Molekulární typizace enterotoxin H-pozitivních kmenů Staphylococcus aureus izolovaných z pacientů a potravin v České republice
• Autoři: RŮŽIČKOVÁ, Vladislava (203 Česká republika, garant), Pavla HOLOCHOVÁ (203 Česká republika), Renata KARPÍŠKOVÁ (203 Česká republika), Roman PANTŮČEK (203 Česká republika), Markéta POSPÍŠILOVÁ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika).
• Vydání: Abstract book. Würzburg, Germany, Pathophysiology of Staphylococci, s. 77-77, 2008.
• Nakladatel: Transregional Collaborative Research Center 34

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Stať ve sborníku
• Obor: Genetika a molekulární biologie
• Stát vydavatele: Německo
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Kód RIV: RIV/00216224:14310/08:00026647
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: Staphylococcus aureus; enterotoxin H; staphylococcal food poisoning; molecular diagnostics
• Štítky: enterotoxin H, Molecular diagnostics, Staphylococcal Food Poisoning, Staphylococcus aureus
• Příznaky: Mezinárodní význam

Anotace

Staphylococcal enterotoxin H (SEH) is a member of the extracellular mature toxin family, secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Thirty-two SEH-positive Staphylococcus aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by detection of the genes for the enterotoxins SEA - SEJ, spa gene polymorphism analysis, pulsed-field gel electrophoresis (PFGE) profiling and ERIC-PCR fingerprinting and prophage carriage. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Our results show that the seh-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.

Anotace česky

Staphylococcal enterotoxin H (SEH) is a member of the extracellular mature toxin family, secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Thirty-two SEH-positive Staphylococcus aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by detection of the genes for the enterotoxins SEA - SEJ, spa gene polymorphism analysis, pulsed-field gel electrophoresis (PFGE) profiling and ERIC-PCR fingerprinting and prophage carriage. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Our results show that the seh-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.

Návaznosti

• MSM0021622415, záměr: Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací