Detailed Information on Publication Record
2008
Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic
RŮŽIČKOVÁ, Vladislava, Pavla HOLOCHOVÁ, Renata KARPÍŠKOVÁ, Roman PANTŮČEK, Markéta POSPÍŠILOVÁ et. al.Basic information
Original name
Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic
Name in Czech
Molekulární typizace enterotoxin H-pozitivních kmenů Staphylococcus aureus izolovaných z pacientů a potravin v České republice
Authors
RŮŽIČKOVÁ, Vladislava (203 Czech Republic, guarantor), Pavla HOLOCHOVÁ (203 Czech Republic), Renata KARPÍŠKOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Markéta POSPÍŠILOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic)
Edition
Abstract book. Würzburg, Germany, Pathophysiology of Staphylococci, p. 77-77, 2008
Publisher
Transregional Collaborative Research Center 34
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/08:00026647
Organization unit
Faculty of Science
Keywords in English
Staphylococcus aureus; enterotoxin H; staphylococcal food poisoning; molecular diagnostics
Tags
International impact
Změněno: 31/10/2008 14:52, prof. RNDr. Roman Pantůček, Ph.D.
V originále
Staphylococcal enterotoxin H (SEH) is a member of the extracellular mature toxin family, secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Thirty-two SEH-positive Staphylococcus aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by detection of the genes for the enterotoxins SEA - SEJ, spa gene polymorphism analysis, pulsed-field gel electrophoresis (PFGE) profiling and ERIC-PCR fingerprinting and prophage carriage. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Our results show that the seh-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.
In Czech
Staphylococcal enterotoxin H (SEH) is a member of the extracellular mature toxin family, secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Thirty-two SEH-positive Staphylococcus aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by detection of the genes for the enterotoxins SEA - SEJ, spa gene polymorphism analysis, pulsed-field gel electrophoresis (PFGE) profiling and ERIC-PCR fingerprinting and prophage carriage. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Our results show that the seh-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.
Links
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