Size, shape and surface morphology of starch granules from
Norway spruce needles revealed by transmission electron
microscopy and atomic force microscopy: effects of elevated CO2
concentration

J 2008

Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration

CABÁLKOVÁ, Jana, Jan PŘIBYL, Petr SKLÁDAL, Pavel KULICH, Josef CHMELÍK et. al.

Basic information

Original name

Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration

Name in Czech

Velikost, tvar a povrchová morfologie škrobových zrn z jehliček smrku obecného dle transmisní elektronové mikroskopie a mikroskopie atomových sil: vliv zvýšené koncentrace CO2

Authors

CABÁLKOVÁ, Jana (203 Czech Republic), Jan PŘIBYL (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, guarantor), Pavel KULICH (203 Czech Republic) and Josef CHMELÍK (203 Czech Republic)

Edition

Tree Physiology, Victoria, Kanada, Heron Publishing, 2008, 0829-318X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Canada

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.283

RIV identification code

RIV/00216224:14310/08:00026653

Organization unit

Faculty of Science

UT WoS

000260027200017

Keywords in English

AFM; Picea abies; starch granules; TEM

Abstract

ORIG CZ

V originále

We compared the effects of ambient (350 ppm) and elevated CO2 concentration (700 ppm) on the size and shape of starch granules in Norway spruce (Picea abies (L.) Karst.) needles during one growing season. Starch granules were isolated from needles by alkaline digestion and analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Measurements made with a particle size analyzer indicated that starch granules ranged between 0.5 and 10 um. Granule size and shape varied according to needle developmental stage and CO2 concentration. Generally, elevated CO2 concentration increased the size of the starch granules. Fine surface structures (less than 10 nm in size) studied by AFM were characterized by the presence of protrusions, furrows and pores.

In Czech

Byly porovnány vlivy normální a zvýšené koncentrace CO2 na škrobová zrna v růstovém období. Izolovaná zrna byla studována mikroskopicky (TEM, AFM), byla provedena analýza rozdělení velikostí zrn.

Links

LC06030, research and development project

Name: Biomolekulární centrum

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

CABÁLKOVÁ, Jana, Jan PŘIBYL, Petr SKLÁDAL, Pavel KULICH and Josef CHMELÍK. Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration. Tree Physiology. Victoria, Kanada: Heron Publishing, 2008, vol. 28, No 10, p. 1593-1599. ISSN 0829-318X.

@article{788775,
   author = {Cabálková, Jana and Přibyl, Jan and Skládal, Petr and Kulich, Pavel and Chmelík, Josef},
   article_location = {Victoria, Kanada},
   article_number = {10},
   keywords = {AFM; Picea abies; starch granules; TEM},
   language = {eng},
   issn = {0829-318X},
   journal = {Tree Physiology},
   note = {IF 2.141 (2007)},
   title = {Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration},
   url = {http://heronpublishing.com/tree/pdf/volume28/28-1593.pdf},
   volume = {28},
   year = {2008}
}

TY  - JOUR
ID  - 788775
AU  - Cabálková, Jana - Přibyl, Jan - Skládal, Petr - Kulich, Pavel - Chmelík, Josef
PY  - 2008
TI  - Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration
JF  - Tree Physiology
VL  - 28
IS  - 10
SP  - 1593-1599
EP  - 1593-1599
PB  - Heron Publishing
SN  - 0829318X
N1  - IF 2.141 (2007)
KW  - AFM
KW  - Picea abies
KW  - starch granules
KW  - TEM
UR  - http://heronpublishing.com/tree/pdf/volume28/28-1593.pdf
N2  - We compared the effects of ambient (350 ppm) and elevated CO2 concentration (700 ppm) on the size and shape of starch granules in Norway spruce (Picea abies (L.) Karst.) needles during one growing season. Starch granules were isolated from needles by alkaline digestion and analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Measurements made with a particle size analyzer indicated that starch granules ranged between 0.5 and 10 um. Granule size and shape varied according to needle developmental stage and CO2 concentration. Generally, elevated CO2 concentration increased the size of the starch granules. Fine surface structures (less than 10 nm in size) studied by AFM were characterized by the presence of protrusions, furrows and pores.
ER  -

CABÁLKOVÁ, Jana, Jan PŘIBYL, Petr SKLÁDAL, Pavel KULICH and Josef CHMELÍK. Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration. \textit{Tree Physiology}. Victoria, Kanada: Heron Publishing, 2008, vol.~28, No~10, p.~1593-1599. ISSN~0829-318X.

Detailed Information on Publication Record