CABÁLKOVÁ, Jana, Jan PŘIBYL, Petr SKLÁDAL, Pavel KULICH and Josef CHMELÍK. Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration. Tree Physiology. Victoria, Kanada: Heron Publishing, 2008, vol. 28, No 10, p. 1593-1599. ISSN 0829-318X.
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Basic information
Original name Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration
Name in Czech Velikost, tvar a povrchová morfologie škrobových zrn z jehliček smrku obecného dle transmisní elektronové mikroskopie a mikroskopie atomových sil: vliv zvýšené koncentrace CO2
Authors CABÁLKOVÁ, Jana (203 Czech Republic), Jan PŘIBYL (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, guarantor), Pavel KULICH (203 Czech Republic) and Josef CHMELÍK (203 Czech Republic).
Edition Tree Physiology, Victoria, Kanada, Heron Publishing, 2008, 0829-318X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Canada
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.283
RIV identification code RIV/00216224:14310/08:00026653
Organization unit Faculty of Science
UT WoS 000260027200017
Keywords in English AFM; Picea abies; starch granules; TEM
Tags AFM, Picea abies, starch granules, TEM
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 29/6/2009 16:27.
Abstract
We compared the effects of ambient (350 ppm) and elevated CO2 concentration (700 ppm) on the size and shape of starch granules in Norway spruce (Picea abies (L.) Karst.) needles during one growing season. Starch granules were isolated from needles by alkaline digestion and analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Measurements made with a particle size analyzer indicated that starch granules ranged between 0.5 and 10 um. Granule size and shape varied according to needle developmental stage and CO2 concentration. Generally, elevated CO2 concentration increased the size of the starch granules. Fine surface structures (less than 10 nm in size) studied by AFM were characterized by the presence of protrusions, furrows and pores.
Abstract (in Czech)
Byly porovnány vlivy normální a zvýšené koncentrace CO2 na škrobová zrna v růstovém období. Izolovaná zrna byla studována mikroskopicky (TEM, AFM), byla provedena analýza rozdělení velikostí zrn.
Links
LC06030, research and development projectName: Biomolekulární centrum
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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