POHANKA, Miroslav, Petr SKLÁDAL and Ota PAVLIŠ. A label free piezoelectric immunosensor for rapid assay of Escherichia coli. Journal of Immunoassay and Immunochemistry. Abingdon, Velká Británie: Taylor and Francis, 2008, vol. 29, No 1, p. 70-79. ISSN 1532-1819.
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Basic information
Original name A label free piezoelectric immunosensor for rapid assay of Escherichia coli
Name in Czech Přímý piezoelektrický imunosensor pro rychlé stanovení Escherichia coli
Authors POHANKA, Miroslav (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, guarantor) and Ota PAVLIŠ (203 Czech Republic).
Edition Journal of Immunoassay and Immunochemistry, Abingdon, Velká Británie, Taylor and Francis, 2008, 1532-1819.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.825
RIV identification code RIV/00216224:14310/08:00026654
Organization unit Faculty of Science
UT WoS 000252219300006
Keywords in English Piezoelectric biosensor; Quartz crystal microbalance; Immunoassay; Immunosensor; Escherichia coli
Tags Escherichia coli, Immunoassay, immunosensor, Piezoelectric biosensor, Quartz crystal microbalance
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 29/6/2009 16:23.
Abstract
piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of the piezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 106-109 CFU/mL; signal of the negative control was not statistically relevant in the selected range. Samples could be analyzed in four minutes and one measuring cycle including regeneration was completed within ten minutes. Repeatability of the developed method is discussed; the signal obtained from three different biosensors was 12.9+/-0.4 Hz for the sample containing 108 CFU/mL.
Abstract (in Czech)
Pro rychlé stanovení (4 min) bakterie Escherichia coli byl zkonstruován piezoelektrický imunosensor.
Links
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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