Detailed Information on Publication Record
2008
Structure-Function Relationships and Engineering of Haloalkane Dehalogenases
DAMBORSKÝ, Jiří, Radka CHALOUPKOVÁ, Martina PAVLOVÁ, Eva CHOVANCOVÁ, Jan BREZOVSKÝ et. al.Basic information
Original name
Structure-Function Relationships and Engineering of Haloalkane Dehalogenases
Name in Czech
Vztah struktutry a funkce a inženýrství haloalkán dehalogenáz.
Authors
DAMBORSKÝ, Jiří (203 Czech Republic, guarantor, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Martina PAVLOVÁ (203 Czech Republic, belonging to the institution), Eva CHOVANCOVÁ (203 Czech Republic, belonging to the institution) and Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution)
Edition
1. vyd. Heidelberg, Handbook of Hydrocarbon and Lipid Microbiology, p. 1081-1098, 18 pp. Handbooks, 1, 2008
Publisher
Springer
Other information
Language
English
Type of outcome
Kapitola resp. kapitoly v odborné knize
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/08:00026663
Organization unit
Faculty of Science
ISBN
978-3-540-77584-3
Keywords in English
QSAR; haloalkan dehalogenases
Tags
Tags
International impact, Reviewed
Změněno: 30/4/2011 11:16, prof. Mgr. Jiří Damborský, Dr.
Abstract
V originále
The structure–function relationships for haloalkane dehalogenases, representing one of the best characterized families of enzymes involved in degradation of halogenated compounds is described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified: (1) activity, (2) thermostability, (3) substrate specificity and (4) enantioselectivity. Some variants carried mutations in the tunnels, connecting the buried active site with surrounding solvent, rather in the active site itself. Mutagenesis in the residues lining the protein tunnels represents a new paradigm in protein engineering.
Links
| LC06010, research and development project |
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| MSM0021622412, plan (intention) |
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