Structure-Function Relationships and Engineering of Haloalkane Dehalogenases

DAMBORSKÝ, Jiří, Radka CHALOUPKOVÁ, Martina PAVLOVÁ, Eva CHOVANCOVÁ and Jan BREZOVSKÝ. Structure-Function Relationships and Engineering of Haloalkane Dehalogenases. In Handbook of Hydrocarbon and Lipid Microbiology. 1st ed. Heidelberg: Springer, 2008, p. 1081-1098. Handbooks, 1. ISBN 978-3-540-77584-3.

Basic information

• Original name: Structure-Function Relationships and Engineering of Haloalkane Dehalogenases
• Name in Czech: Vztah struktutry a funkce a inženýrství haloalkán dehalogenáz.
• Authors: DAMBORSKÝ, Jiří (203 Czech Republic, guarantor, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Martina PAVLOVÁ (203 Czech Republic, belonging to the institution), Eva CHOVANCOVÁ (203 Czech Republic, belonging to the institution) and Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution).
• Edition: 1. vyd. Heidelberg, Handbook of Hydrocarbon and Lipid Microbiology, p. 1081-1098, 18 pp. Handbooks, 1, 2008.
• Publisher: Springer

Other information

• Original language: English
• Type of outcome: Chapter(s) of a specialized book
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/08:00026663
• Organization unit: Faculty of Science
• ISBN: 978-3-540-77584-3
• Keywords in English: QSAR; haloalkan dehalogenases
• Tags: haloalkan dehalogenases, QSAR
• Tags: International impact, Reviewed

Abstract

The structure–function relationships for haloalkane dehalogenases, representing one of the best characterized families of enzymes involved in degradation of halogenated compounds is described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified: (1) activity, (2) thermostability, (3) substrate specificity and (4) enantioselectivity. Some variants carried mutations in the tunnels, connecting the buried active site with surrounding solvent, rather in the active site itself. Mutagenesis in the residues lining the protein tunnels represents a new paradigm in protein engineering.

Links

• LC06010, research and development project: Name: Centrum biokatalýzy a biotransformací

Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)