VAŘECHA, Miroslav, Pavel MATULA and Michal KOZUBEK. New approaches to automated cell image acquisition and analysis using fluorescence microscopy. In XIX. Biologické Dny - International Symposium "The Functional Organization of the Cell Nucleus". 2008. ISBN 978-80-7399-545-4.
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Basic information
Original name New approaches to automated cell image acquisition and analysis using fluorescence microscopy
Name in Czech Nové přístupy k automatizovanému snímání a analýze buněk pomocí fluorescenční mikroskopie
Authors VAŘECHA, Miroslav (203 Czech Republic, guarantor), Pavel MATULA (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic).
Edition XIX. Biologické Dny - International Symposium "The Functional Organization of the Cell Nucleus" 2008.
Other information
Original language English
Type of outcome Requested lectures
Field of Study 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14330/08:00026665
Organization unit Faculty of Informatics
ISBN 978-80-7399-545-4
Keywords in English live-cell imaging;fluorescence microscopy;image analysis;
Tags fluorescence microscopy, Image analysis, live-cell imaging
Tags International impact, Reviewed
Changed by Changed by: Mgr. Miroslav Vařecha, Ph.D., učo 82780. Changed: 2/6/2010 11:52.
Abstract
Fluorescence microscopy has become the leading technology to study structure as well as dynamics of cellular components. The studies can be performed in three-dimensional (3D) spatial coordinate system as well as in time and spectral dimensions. For example, in cell nucleus studies, fluorescence microscopy enables to observe not only 3D chromatin structure (especially spatial organization of selected genes or chromosomes) but also its dynamics in space (time-lapse imaging of fluorescence-labeled histones within cell nucleus). At the Centre for Biomedical Image Analysis of Masaryk University in Brno, we have been developing special systems for automated cell image acquisition and analysis using fluorescence microscopy. We have called them high-resolution image cytometers (HRCMs) in literature. The presentation will focus on the latest developments in HRCM technology. Besides microscopy hardware and software, also examples of possible applications will be presented.
Abstract (in Czech)
Nové přístupy k automatizovanému snímání a analýze buněk pomocí fluorescenční mikroskopie.
Links
LC535, research and development projectName: Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii
Investor: Ministry of Education, Youth and Sports of the CR, Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii
MSM0021622419, plan (intention)Name: Vysoce paralelní a distribuované výpočetní systémy
Investor: Ministry of Education, Youth and Sports of the CR, Highly Parallel and Distributed Computing Systems
2B06052, research and development projectName: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)
Investor: Ministry of Education, Youth and Sports of the CR, Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images
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