2008
METALLOTHIONEIN-CISPLATIN INTERACTION REVEALED BY QUARTZ CRYSTAL AND MERCURY ELECTRODE
KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Violetta SHESTIVSKA, Tomáš ECKSCHLAGER, Jan HRABĚTA et. al.Basic information
Original name
METALLOTHIONEIN-CISPLATIN INTERACTION REVEALED BY QUARTZ CRYSTAL AND MERCURY ELECTRODE
Name in Czech
Interakce metalothioneinu s cisplatinou odhalené pomocí křemíkového krystalu a rtuťové elektrody
Authors
KŘÍŽKOVÁ, Soňa (203 Czech Republic), Ivo FABRIK (203 Czech Republic), Violetta SHESTIVSKA (203 Czech Republic), Tomáš ECKSCHLAGER (203 Czech Republic), Jan HRABĚTA (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)
Edition
Brno, Electrochemistry of Nucleic Acids and Proteins. Book of Abstracts, p. 78-78, 1 pp. 2008
Publisher
Biofyzikální ústav AV ČR, v.v.i.
Other information
Language
English
Type of outcome
Proceedings paper
Field of Study
10405 Electrochemistry
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
RIV identification code
RIV/00216224:14310/08:00024315
Organization unit
Faculty of Science
Keywords in English
cisplatin; metallothionein; differential pulse voltammetry; quartz crystal
Changed: 10/11/2008 13:31, prof. RNDr. Libuše Trnková, CSc.
V originále
The aim of this work was to investigate the interactions of metallothioneins with cisplatin. For this purpose we employed adsorptive transfer stripping technique with differential pulse voltammetry (mercury electrode) and gravimetric measurements (quartz crystal). Primarily we optimized experimental conditions for determination of MT using both techniques. The average concentration of the drug bound to DNA was estimated as 8.1 ng of cisplatin per 500 ng of DNA by voltammetry. Gravimetric measurements gave very similar results.
In Czech
Cílem této práce bylo studium interakcí metalothioneinů (MT) s cisplatinou. K tomu byla využia adsorptivní přenosová rozpouštěcí technika s diferenční pulsní voltametrií (rtuťová elektroda) a gravimetrické měření. Nejprve jsme optimalizovali experimentální podmínky pro stanovení MT oběma metodami. Průměrná koncentrace cis-Pt vázané na DNA byla voltametricky stanovena na 8.1 ng cis-Pt na 500 ng DNA. Gravimetrické měření mělo velmi podobné výsledky.
Links
| IAA401990701, research and development project |
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