Detailed Information on Publication Record
2008
A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction
FABRIK, Ivo, Zuzana RUFEROVÁ, Klára HILSCHEROVÁ, Vojtěch ADAM, Libuše TRNKOVÁ et. al.Basic information
Original name
A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction
Name in Czech
Stanovení metalothioneinu v larvách pakomára (Chironomus riparius) s využitím Brdičkovy reakce
Authors
FABRIK, Ivo (203 Czech Republic, guarantor), Zuzana RUFEROVÁ (203 Czech Republic), Klára HILSCHEROVÁ (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic) and René KIZEK (203 Czech Republic)
Edition
Sensors, Basel Switzerland, MOLECULAR DIVERSITY PRESERVATION INT, 2008, 1424-8220
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10405 Electrochemistry
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.870
RIV identification code
RIV/00216224:14310/08:00026700
Organization unit
Faculty of Science
UT WoS
000258180500003
Keywords (in Czech)
elektrochemie, těžké kovy, metalothionein
Keywords in English
electrochemistry; heavy metals;metallothionein
Tags
International impact, Reviewed
Změněno: 25/6/2009 09:47, prof. RNDr. Luděk Bláha, Ph.D.
V originále
Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothionein and total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from field studies using differential pulse voltammetry Brdicka reaction. Unique electrochemical instrument, stationary electrochemical analyser Autolab coupled with autosampler, was utilized for the analysis of the samples. The detection limit for MT was evaluated as 5 nM. The larvae exposed to two doses of cadmium(II) ions for fifteen days under laboratory controlled conditions were at the end of the exposure killed, homogenized and analysed. MT content in control samples was 1.2 microM, in larvae exposed to 50 ng Cd/g it was 2.0 microM and in larvae exposed to 50 microg Cd/g 2.9 microM. Moreover at field study chironomid larvae as well as sediment samples have been collected from eight field sites with different levels of pollution by heavy. The metals content (chromium, nickel, copper, zinc, arsenic, molybdenum,cadmium, tin and lead) in the sediment and or MT content in the chironomid larvae were determined by inductively coupled plasma mass spectrometry or Brdicka reaction, respectively.
In Czech
Mezi biomolekuly, které odpovídají na stres, patří metalothioneiny, vhodné pro sledování kontaminace těžkými kovy. Cílem této práce bylo stanovit obsah metalothioneinů a celkových thiolů v larvách pakomárů exponovaných kadmiem a přírodními sedimenty.
Links
| MSM0021622412, plan (intention) |
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