Employment of electrochemical techniques for metallothionein
determination in tumor cell lines and patients with a tumor
disease

J 2008

Employment of electrochemical techniques for metallothionein determination in tumor cell lines and patients with a tumor disease

FABRIK, Ivo, Soňa KŘÍŽKOVÁ, Dalibor HÚSKA, Vojtěch ADAM, Jaromír HUBÁLEK et. al.

Basic information

Original name

Employment of electrochemical techniques for metallothionein determination in tumor cell lines and patients with a tumor disease

Name in Czech

Využití elektrochemických metod pro stanovení metallothioneinu v nádorových buněčných liniích a u pacientů s nádorovým onemocněním

Authors

FABRIK, Ivo (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic), Dalibor HÚSKA (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Jaromír HUBÁLEK (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic), Tomáš ECKSCHLAGER (203 Czech Republic), Jiří KUKAČKA (203 Czech Republic), Richard PRŮŠA (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)

Edition

Electroanalysis, Weinheim, Germany, Wiley VCH Verlag GmbH, 2008, 1040-0397

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.901

RIV identification code

RIV/00216224:14310/08:00028433

Organization unit

Faculty of Science

UT WoS

000258087300003

Keywords in English

chromatography; tumor cells; electrochemistry; metallothionein

Abstract

ORIG CZ

V originále

In the present paper we employed adsorptive transfer stripping technique coupled with chronopotentiometric stripping analysis for determination of metallothionein (MT) in tumor cell lines and differential pulse voltammetry Brdicka reaction for determination of MT in blood serum of patients with head and neck cancer or retinoblastoma, and of rats treated with cisplatin with respect to discuss the role of MT in formation of resistance on treatment with heavy metal based cytostatics. The cisplatin or carboplatin sensitive and resistant neuroblastoma cell lines were derived from the maternal cell line isolated from the bone metastasis of patients with neuroblastoma. Based on the results obtained it can be concluded that level of MT increases with higher dose of platinum based cytostatics at cells. Further we focused on determination of MT in blood serum of rats treated with cisplatin (two doses 1.05 mg and/or 2.1 mg of cisplatin per kg). The highest level of MT at rats treated with 1.05 mg cisplatin was determined after four hours as 4.9 mol/L. In the case of the second experimental group the maximum was reached even after two hours of the treatment as 4.8 mol/L. In addition we were interested in the effect of cisplatin or carboplatin treatment of patients with a tumor disease. At patients with tumor in head and neck area treated with cisplatin we observed that the level of MT was going higher due to administration of the drug. This phenomenon was observed at all patients. However at patients with retinoblastoma treated with carboplatin we observed various phenomena including decreasing, increasing or no changes in MT level. Progression of MT levels was therefore individual and probably depended on tumor resistance to carboplatin.

In Czech

Stanovení metallothioneinu v nádorových buněčných liniích a v séru pacientů.

Links

IAA401990701, research and development project

Name: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu

Citovat

FABRIK, Ivo, Soňa KŘÍŽKOVÁ, Dalibor HÚSKA, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ, Tomáš ECKSCHLAGER, Jiří KUKAČKA, Richard PRŮŠA and René KIZEK. Employment of electrochemical techniques for metallothionein determination in tumor cell lines and patients with a tumor disease. Electroanalysis. Weinheim, Germany: Wiley VCH Verlag GmbH, 2008, vol. 20, No 14, p. 1521-1532, 11 pp. ISSN 1040-0397.

@article{791484,
   author = {Fabrik, Ivo and Křížková, Soňa and Húska, Dalibor and Adam, Vojtěch and Hubálek, Jaromír and Trnková, Libuše and Eckschlager, Tomáš and Kukačka, Jiří and Průša, Richard and Kizek, René},
   article_location = {Weinheim, Germany},
   article_number = {14},
   keywords = {chromatography; tumor cells; electrochemistry; metallothionein},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Employment of electrochemical techniques for metallothionein determination in tumor cell lines and patients with a tumor disease},
   volume = {20},
   year = {2008}
}

TY  - JOUR
ID  - 791484
AU  - Fabrik, Ivo - Křížková, Soňa - Húska, Dalibor - Adam, Vojtěch - Hubálek, Jaromír - Trnková, Libuše - Eckschlager, Tomáš - Kukačka, Jiří - Průša, Richard - Kizek, René
PY  - 2008
TI  - Employment of electrochemical techniques for metallothionein determination in tumor cell lines and patients with a tumor disease
JF  - Electroanalysis
VL  - 20
IS  - 14
SP  - 1521-1532
EP  - 1521-1532
PB  - Wiley VCH Verlag GmbH
SN  - 10400397
KW  - chromatography
KW  - tumor cells
KW  - electrochemistry
KW  - metallothionein
N2  - In the present paper we employed adsorptive transfer stripping technique coupled with chronopotentiometric stripping analysis for determination of metallothionein (MT) in tumor cell lines and differential pulse voltammetry Brdicka reaction for determination of MT in blood serum of patients with head and neck cancer or retinoblastoma, and of rats treated with cisplatin with respect to discuss the role of MT in formation of resistance on treatment with heavy metal based cytostatics. The cisplatin or carboplatin sensitive and resistant neuroblastoma cell lines were derived from the maternal cell line isolated from the bone metastasis of patients with neuroblastoma. Based on the results obtained it can be concluded that level of MT increases with higher dose of platinum based cytostatics at cells. Further we focused on determination of MT in blood serum of rats treated with cisplatin (two doses 1.05 mg and/or 2.1 mg of cisplatin per kg). The highest level of MT at rats treated with 1.05 mg cisplatin was determined after four hours as 4.9 mol/L. In the case of the second experimental group the maximum was reached even after two hours of the treatment as 4.8 mol/L. In addition we were interested in the effect of cisplatin or carboplatin treatment of patients with a tumor disease. At patients with tumor in head and neck area treated with cisplatin we observed that the level of MT was going higher due to administration of the drug. This phenomenon was observed at all patients. However at patients with retinoblastoma treated with carboplatin we observed various phenomena including decreasing, increasing or no changes in MT level. Progression of MT levels was therefore individual and probably depended on tumor resistance to carboplatin.
ER  -

FABRIK, Ivo, Soňa KŘÍŽKOVÁ, Dalibor HÚSKA, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ, Tomáš ECKSCHLAGER, Jiří KUKAČKA, Richard PRŮŠA and René KIZEK. Employment of electrochemical techniques for metallothionein determination in tumor cell lines and patients with a tumor disease. \textit{Electroanalysis}. Weinheim, Germany: Wiley VCH Verlag GmbH, 2008, vol.~20, No~14, p.~1521-1532, 11 pp. ISSN~1040-0397.

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