An electrochemical detection of metallothioneins at the
zeptomole level in nanolitre volumes

J 2008

An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes

ADAM, Vojtěch, Jiří BALOUN, Ivo FABRIK, Libuše TRNKOVÁ, René KIZEK et. al.

Basic information

Original name

An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes

Name in Czech

Elektrochemická detekce metalothioneinů v zeptomolárních množstvích v nanolitrových objemech

Authors

ADAM, Vojtěch (203 Czech Republic), Jiří BALOUN (203 Czech Republic), Ivo FABRIK (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)

Edition

Sensors, Basel, Switzerland, MOLECULAR DIVERSITY PRESERVATION INT, 2008, 1424-8220

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.870

RIV identification code

RIV/00216224:14310/08:00028434

Organization unit

Faculty of Science

UT WoS

000256642400013

Keywords in English

metallothionein; voltammetry; biosensor

Abstract

ORIG CZ

V originále

We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer. Based on the results obtained it can be concluded that the improved technique can be used to detect a thiolprotein in very low sample volumes and can also prevent interferences during the washing and transferring step.

In Czech

Vylepšení metody adsorptivní přenosové rozpouštěcí techniky ve spojení s diferenční pulsní voltametrií ke stanovení thiolových proteinů.

Links

IAA401990701, research and development project

Name: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu

Citovat

ADAM, Vojtěch, Jiří BALOUN, Ivo FABRIK, Libuše TRNKOVÁ and René KIZEK. An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes. Sensors. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, vol. 8, No 4, p. 2293-2305, 12 pp. ISSN 1424-8220.

@article{791493,
   author = {Adam, Vojtěch and Baloun, Jiří and Fabrik, Ivo and Trnková, Libuše and Kizek, René},
   article_location = {Basel, Switzerland},
   article_number = {4},
   keywords = {metallothionein; voltammetry; biosensor},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes},
   volume = {8},
   year = {2008}
}

TY  - JOUR
ID  - 791493
AU  - Adam, Vojtěch - Baloun, Jiří - Fabrik, Ivo - Trnková, Libuše - Kizek, René
PY  - 2008
TI  - An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes
JF  - Sensors
VL  - 8
IS  - 4
SP  - 2293-2305
EP  - 2293-2305
PB  - MOLECULAR DIVERSITY PRESERVATION INT
SN  - 14248220
KW  - metallothionein
KW  - voltammetry
KW  - biosensor
N2  - We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer. Based on the results obtained it can be concluded that the improved technique can be used to detect a thiolprotein in very low sample volumes and can also prevent interferences during the washing and transferring step.
ER  -

ADAM, Vojtěch, Jiří BALOUN, Ivo FABRIK, Libuše TRNKOVÁ and René KIZEK. An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes. \textit{Sensors}. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, vol.~8, No~4, p.~2293-2305, 12 pp. ISSN~1424-8220.

Detailed Information on Publication Record