Utilizing of adsorptive transfer stripping technique Brdicka
reaction for determination of metallothioneins level in
melanoma cells, blood serum and tissues

J 2008

Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues

KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Vojtěch ADAM, Jiří KUKAČKA, Richard PRŮŠA et. al.

Basic information

Original name

Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues

Name in Czech

Využití adsorptivní přenosové rozpouštěcí techniky Brdičkovy reakce pro stanovení metalothioneinů v melanomových buňkách, krevním séru a tkáních

Authors

KŘÍŽKOVÁ, Soňa (203 Czech Republic), Ivo FABRIK (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Jiří KUKAČKA (203 Czech Republic), Richard PRŮŠA (203 Czech Republic), Grace J. CHAVIS (840 United States of America), Libuše TRNKOVÁ (203 Czech Republic), Jan STRNADEL (203 Czech Republic), Vratislav HORÁK (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)

Edition

Sensors, Basel, Switzerland, MOLECULAR DIVERSITY PRESERVATION INT, 2008, 1424-8220

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.870

RIV identification code

RIV/00216224:14310/08:00028435

Organization unit

Faculty of Science

UT WoS

000257248800012

Keywords in English

melanoma; metallothionein; electrochemistry

Abstract

ORIG CZ

V originále

In the paper we utilized the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. Primarily we attempted to investigate the influence of dilution of real sample on MT electrochemical response. Dilution of samples of 1 000 times was chosen the most suitable for determination of MT level in biological samples. Then we quantified the MT level in the melanoma cells, the animal melanoma tissues and the blood serum samples. The MT content in the cells varied within the range from 4.2 to 11.2 microM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 micro g of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover the average MT level in the blood serum samples from patients with melanoma was 3.0 +- 0.8 microM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum.

In Czech

Využití adsorptivní přenosové rozpouštěcí diferenční pulsní voltametrie ke stanovení metalothioneinů v melanomových buňkách, zvířecích melanomových tkáních a v séru pacientů se zhoubným melanomem.

Links

IAA401990701, research and development project

Name: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu

Citovat

KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Vojtěch ADAM, Jiří KUKAČKA, Richard PRŮŠA, Grace J. CHAVIS, Libuše TRNKOVÁ, Jan STRNADEL, Vratislav HORÁK and René KIZEK. Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues. Sensors. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, vol. 8, No 5, p. 3106-3122, 16 pp. ISSN 1424-8220.

@article{791494,
   author = {Křížková, Soňa and Fabrik, Ivo and Adam, Vojtěch and Kukačka, Jiří and Průša, Richard and Chavis, Grace J. and Trnková, Libuše and Strnadel, Jan and Horák, Vratislav and Kizek, René},
   article_location = {Basel, Switzerland},
   article_number = {5},
   keywords = {melanoma; metallothionein; electrochemistry},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues},
   volume = {8},
   year = {2008}
}

TY  - JOUR
ID  - 791494
AU  - Křížková, Soňa - Fabrik, Ivo - Adam, Vojtěch - Kukačka, Jiří - Průša, Richard - Chavis, Grace J. - Trnková, Libuše - Strnadel, Jan - Horák, Vratislav - Kizek, René
PY  - 2008
TI  - Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues
JF  - Sensors
VL  - 8
IS  - 5
SP  - 3106-3122
EP  - 3106-3122
PB  - MOLECULAR DIVERSITY PRESERVATION INT
SN  - 14248220
KW  - melanoma
KW  - metallothionein
KW  - electrochemistry
N2  - In the paper we utilized the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. Primarily we attempted to investigate the influence of dilution of real sample on MT electrochemical response. Dilution of samples of 1 000 times was chosen the most suitable for determination of MT level in biological samples. Then we quantified the MT level in the melanoma cells, the animal melanoma tissues and the blood serum samples. The MT content in the cells varied within the range from 4.2 to 11.2 microM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 micro g of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover the average MT level in the blood serum samples from patients with melanoma was 3.0 +- 0.8 microM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum.
ER  -

KŘÍŽKOVÁ, Soňa, Ivo FABRIK, Vojtěch ADAM, Jiří KUKAČKA, Richard PRŮŠA, Grace J. CHAVIS, Libuše TRNKOVÁ, Jan STRNADEL, Vratislav HORÁK and René KIZEK. Utilizing of adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues. \textit{Sensors}. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, vol.~8, No~5, p.~3106-3122, 16 pp. ISSN~1424-8220.

Detailed Information on Publication Record