Constant current chronopotentiometry and voltammetry of native and denatured serum albumin at mercury and carbon electrodes

OSTATNÁ, Veronika, Filiz KURALAY, Libuše TRNKOVÁ and Emil PALEČEK. Constant current chronopotentiometry and voltammetry of native and denatured serum albumin at mercury and carbon electrodes. Electroanalysis. Weinheim, Germany: Wiley-VCH Verlag GmbH, 2008, vol. 20, No 13, p. 1406-1413. ISSN 1040-0397.

Basic information

Original name

Constant current chronopotentiometry and voltammetry of native and denatured serum albumin at mercury and carbon electrodes

Name in Czech

Chronopotenciometrie a voltametrie nativního a denaturovaného sérového albuminu na rtuťových a uhlíkových elektrodách

Authors

OSTATNÁ, Veronika (703 Slovakia), Filiz KURALAY (792 Turkey), Libuše TRNKOVÁ (203 Czech Republic, guarantor) and Emil PALEČEK (203 Czech Republic)

Edition

Electroanalysis, Weinheim, Germany, Wiley-VCH Verlag GmbH, 2008, 1040-0397

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.901

RIV identification code

RIV/00216224:14310/08:00034074

Organization unit

Faculty of Science

UT WoS

000257618300003

Keywords in English

chronopotentiometry; voltammetry; albumin

Tags

albumin, chronopotentiometry, Voltammetry

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Abstract

V originále

Constant current chronopotentiometric peak H at mercury electrodes was recently shown as a sensitive tool for global and local changes in protein conformation. Large differences between the heights of peak H of native (hBSAnat) and denatured BSA (hBSAden) were observed. The ratio hBSAden/hBSAnat increased with more negative stripping current suggesting that the rate of potential change is important for discrimination between native and denatured BSA. Voltammetric peaks of BSA were less well developed and BSAden/BSAnat was much smaller. It was not possible to discriminate BSAden and BSAnat using carbon electrodes.

In Czech

Chronopotenciometrický pík H na rtuťové elektrodě se nedávno ukázal jako citlivý nástroj pro studium globálních i lokálních změn v konformaci proteinů. Byly pozorovány velké rozdíly ve výšce píku nativního a denaturovaného albuminu.

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Links

LC06035, research and development project	Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu. Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
OC 174, research and development project	Name: Využití rozšířených elektrochemických metod ke studiu elektrochemických a bioelektrochemických procesů na mezifázi elektroda/elektrolyt Investor: Ministry of Education, Youth and Sports of the CR, Utilization of Hyphenated Electrochemical Methods to the Study of Electrochemical and Bio-electrochemical Processes at Solid-Electrolyte Interfaces