Detailed Information on Publication Record
2008
Mus81/Mms4 nuclease and cleavage of replication/recombination intermediates
KREJČÍ, LumírBasic information
Original name
Mus81/Mms4 nuclease and cleavage of replication/recombination intermediates
Name in Czech
Mus81/Mms4 endonukleáza a štěpení replikačníci/rekombinačních meziproduktů
Authors
Edition
Portugalsko, 2008
Publisher
HHMI
Other information
Language
English
Type of outcome
Audiovizuální tvorba
Field of Study
10600 1.6 Biological sciences
Country of publisher
Portugal
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Organization unit
Faculty of Science
Keywords in English
DNA repair; DNA damage; replication; genomic instability
Tags
International impact
Změněno: 31/3/2010 11:37, doc. Mgr. Lumír Krejčí, Ph.D.
V originále
DNA replication is typically highly processive mechanism with astonishing precision, despite the fact that it frequently encounters barriers caused by endogenous and exogenous genotoxic agents. It is not surprising that DNA replication does not act alone, but operate in coordination with recombination, and DNA repair processes to overcame such replication obstacles, ensure cellular viability and achieve genomic stability. Several mechanisms by which replication forks can be restarted following arrest have been described with Mus81/Mms4 complex, possessing structure specific endonuclease activity, being one of them. The mus81 mutant shows synthetic interaction with mutations in replication proteins and hypersensitivity genotoxic reagents that in general cause replication fork stalling and collapse. In addition, Mus81 is also known to play role in normal processing of DNA lesion and its inactivation result in elevated levels of genomic instability, elicits checkpoint activation and checkpoint-dependent cell cycle arrest. Our study shows direct association between Rad54 and Mus81 using purified proteins as well as dramatic stimulation of Mus81/Mms4 activity by Rad54 protein on variety of substrates. In addition, we provide evidence that ATP binding and hydrolysis is not required for this stimulation and Rad54 is able to target Mus81/Mms4 complex to its substrates. We propose that Rad54 together with Mus81/Mms4 protein is involved in processing DNA substrates that are intermediates arising during DNA replication and recombination.
In Czech
Charakterizace strukturně-specifické nukleázy Mus81/MMS4 při rozpouštění rekombinačních a replikačních meziproduktů.
Links
| LC06030, research and development project |
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| ME 888, research and development project |
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| MSM0021622413, plan (intention) |
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