| HOLČÁKOVÁ, Jitka, Lenka HERNYCHOVÁ, Pavel BOUCHAL, Kristýna BROŽKOVÁ, Jan ŽALOUDÍK, Dalibor VALÍK, Rudolf NENUTIL and Bořivoj VOJTĚŠEK. Identification of alphaB-crystallin, a biomarker of renal cell carcinoma by SELDI-TOF MS. The International Journal of Biological Markers. Itálie: Wichtig editore, 2008, vol. 23, No 1, p. 48-53. ISSN 0393-6155. |
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@article{792441,
author = {Holčáková, Jitka and Hernychová, Lenka and Bouchal, Pavel and Brožková, Kristýna and Žaloudík, Jan and Valík, Dalibor and Nenutil, Rudolf and Vojtěšek, Bořivoj},
article_location = {Itálie},
article_number = {1},
keywords = {alphaB-crystallin; biomarker; ProteinChip; renal cell carcionoma; SELDI-TOF MS},
language = {eng},
issn = {0393-6155},
journal = {The International Journal of Biological Markers},
title = {Identification of alphaB-crystallin, a biomarker of renal cell carcinoma by SELDI-TOF MS},
volume = {23},
year = {2008}
}
TY - JOUR
ID - 792441
AU - Holčáková, Jitka - Hernychová, Lenka - Bouchal, Pavel - Brožková, Kristýna - Žaloudík, Jan - Valík, Dalibor - Nenutil, Rudolf - Vojtěšek, Bořivoj
PY - 2008
TI - Identification of alphaB-crystallin, a biomarker of renal cell carcinoma by SELDI-TOF MS
JF - The International Journal of Biological Markers
VL - 23
IS - 1
SP - 48-53
EP - 48-53
PB - Wichtig editore
SN - 03936155
KW - alphaB-crystallin
KW - biomarker
KW - ProteinChip
KW - renal cell carcionoma
KW - SELDI-TOF MS
N2 - Spectrometric-based surface-enhanced laser desorption/ionization ProteinChip (SELDI-TOF) facilitates rapid and easy analysis of protein mixtures and is often exploited to define potential diagnostic markers from sera. However, SELDI-TOF is a relatively insensitive technique and unable to detect circulating proteins at low levels even if they are differentially expressed in cancer patients. Therefore, we applied this technology to study tissues from renal cell carcinomas (RCC) in comparison to healthy controls. We found that different biomarkers are identified from tissues than those previously identified in serum, and that serum markers are often not produced by the tumors themselves at detectable levels, reflecting the nonspecific nature of many circulating biomarkers. We detected and characterized alphaB-crystallin as an overexpressed protein in RCC tissues and showed differential expression by immunohistochemistry.
ER -
HOLČÁKOVÁ, Jitka, Lenka HERNYCHOVÁ, Pavel BOUCHAL, Kristýna BROŽKOVÁ, Jan ŽALOUDÍK, Dalibor VALÍK, Rudolf NENUTIL and Bořivoj VOJTĚŠEK. Identification of alphaB-crystallin, a biomarker of renal cell carcinoma by SELDI-TOF MS. \textit{The International Journal of Biological Markers}. Itálie: Wichtig editore, 2008, vol.~23, No~1, p.~48-53. ISSN~0393-6155.
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