MATULOVÁ, Petra. Role of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates . (ole of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates .). 2008. |
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Basic information | |
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Original name | Role of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates . |
Name in Czech | Role SRS2 a Mus81/MMS4 v procesu recombinace a replikace. |
Authors | MATULOVÁ, Petra (203 Czech Republic, guarantor). |
Edition | 2008. |
Other information | |
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Original language | English |
Type of outcome | Audiovisual works |
Field of Study | 10600 1.6 Biological sciences |
Country of publisher | Austria |
Confidentiality degree | is not subject to a state or trade secret |
WWW | URL |
RIV identification code | RIV/00216224:14310/08:00026760 |
Organization unit | Faculty of Science |
Keywords in English | DNA repair; homologous recombination; nuclease activity; MUS81; SRS2 |
Tags | DNA repair, HOMOLOGOUS RECOMBINATION, MUS81, nuclease activity, Srs2 |
Tags | International impact |
Changed by | Changed by: RNDr. Petra Matulová, CSc., učo 9032. Changed: 14/11/2008 16:24. |
Abstract |
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Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific. |
Abstract (in Czech) |
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Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific. |
Links | |
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MSM 143100005, plan (intention) | Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu |
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism | |
MSM0021622413, plan (intention) | Name: Proteiny v metabolismu a při interakci organismů s prostředím |
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment |