MATULOVÁ, Petra, Veronika ALTMANNOVÁ, María Victoria MARINI PALOMEQUE and Lumír KREJČÍ. Význam proteinů Srs2 a Mus81/MMS4 během opravy DNA. (Role of Srs2 and Mus81/MMS4 in processing recombination/replication intermediates). 2008.
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Basic information
Original name Význam proteinů Srs2 a Mus81/MMS4 během opravy DNA.
Name in Czech Význam proteinů Srs2 a Mus81/MMS4 během opravy DNA.
Authors MATULOVÁ, Petra, Veronika ALTMANNOVÁ, María Victoria MARINI PALOMEQUE and Lumír KREJČÍ.
Edition 2008.
Other information
Original language English
Type of outcome Audiovisual works
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Organization unit Faculty of Science
Keywords in English DNA repair; homologous recombination; nuclease activity; MUS81; SRS2
Tags DNA repair, HOMOLOGOUS RECOMBINATION, MUS81, nuclease activity, Srs2
Tags International impact
Changed by Changed by: RNDr. Petra Matulová, CSc., učo 9032. Changed: 14/11/2008 16:46.
Abstract
Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific
Abstract (in Czech)
Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific
Links
MSM 143100005, plan (intention)Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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