Detailed Information on Publication Record
2008
Význam proteinů Srs2 a Mus81/MMS4 během opravy DNA.
MATULOVÁ, Petra, Veronika ALTMANNOVÁ, María Victoria MARINI PALOMEQUE and Lumír KREJČÍBasic information
Original name
Význam proteinů Srs2 a Mus81/MMS4 během opravy DNA.
Name in Czech
Význam proteinů Srs2 a Mus81/MMS4 během opravy DNA.
Authors
Edition
2008
Other information
Language
English
Type of outcome
Audiovizuální tvorba
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Organization unit
Faculty of Science
Keywords in English
DNA repair; homologous recombination; nuclease activity; MUS81; SRS2
Tags
International impact
Změněno: 14/11/2008 16:46, RNDr. Petra Matulová, CSc.
V originále
Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific
In Czech
Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific
Links
| MSM 143100005, plan (intention) |
| ||
| MSM0021622413, plan (intention) |
|